Biofilm and antibiotic resistance profile among Pseudomonas aeruginosa isolated from clinical samples
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 1, pp. 1135-1139
  • Published Online: 28 May 2020
  • Open Access Full Text (PDF)


Pseudomonas aeruginosa is an opportunistic gram-negative bacilli capable of causing a wide range of problematic acute and chronic infections. It is survival, adaptation and struggle to numerous classes of antibiotics pushing it as life threating pathogen. Sixty P. aeruginosa isolates were recovered from 7 types of specimens (1 Pharyngeal swab, 1 CSF, 2 High vaginal swab, 11 ear swab,12 wound swab, 12 Broncoalveolar lavage and 21 midstream urine). All isolates confirmed by Pseudomonas chromogenic agar Biofilm formation were assayed by TCP while antibiotic susceptibility performed according to CLSI-2019. The results of isolation revealed high percentage of P. aeruginosa among cystitis patients 21/60(35%), lower respiratory tract infection patients 12/60(20%), wounds infection 12/60(20%) and otitis media 11/60(18.3%) while 2/60(3.3%) for bacterial vaginosis, 1/60(1.7%) for each of meningitis and pharyngitis. The results of Biofilm formation using TCP method revealed that 51/60 (85%) were biofilm former while 9/60 (15%) were non biofilm former. Concern correlation of biofilm-formation with antibiotic resistance, the results showed strong association between biofilm and resistance to Beta-lactams. The P. aeruginosa resistance to Cefepime 55/60(91.7%), Piperacillin 44/60 (73.3%) and Ceftazidime 43/60(71.7%) while weak association were seen for Aztreonam resistance 21/60 (35%). Carbapenem antibiotics also show weak correlation between biofilm and antibiotic-resistance in which only 11/60 (18.3%) and 9/60 (15%) of isolates were resist meropenem and imipenem respectively. Fluoroquinolone resistance were 31/60 (51.7%), 29/60 (48.3%), 21/60(35%) and 18/60 (30%) for lomefloxacin, ciprofloxacin, ofloxacin and levofloxacin respectively. Totally MDR P. aeruginosa compile 38 (63.34%) while non-MDR P. aeruginosa consist 22(36.66%). The most prevalent phenotype is BETA/AMINO/FLUORO which compile 13(21.66%). Concern relationship of multidrug resistance with biofilm formation the results revealed that 21/51 (41.17%) were non-MDR while 30/51(58.83%) were MDR. There is a strong correlation between biofilm formation and resistance to antibiotic among P. aeruginosa recovered from clinical samples with dominance of coexistence resistance among MDR isolates.


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