Assessment of enzyme polyamine oxidase in patients with chronic kidney disease
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 1, pp. 1893-1899
  • Published Online: 30 Jun 2020
  • Open Access Full Text (PDF)

Abstract

The present research involves assessment of polyamine oxidase in chronic kidney disease (CKD) using different biochemical techniques. The study involved 25 outpatient, of both genders, attended state hospitals in Mosul province complaining kidney failure. Three proteinaceous material were separated by ion exchange chromatography from precipitate produced by ammonium sulfate saturation. A significant increase (p≤0.01) in the activity of polyamine oxidase was detected in comparison with similar number of healthy individual used as control. The first and second out of four peaks had high activity of enzyme polyamine oxidase. A single peak of enzyme polyamine oxidase was detected, using SDS-electrophoresis gel filtration, with molecular weight of 56,000 Dalton. Using 240m mole/l of Spermine-tetra-hydrochloride as a substrate for enzyme, (100 m Tris-HCl Buffer mole /l ) at pH 7.5 for 5 minutes at 450C a maximum activity for polyamine oxidase was obtained. However, with Line-Weaver-Burk plot the maximum velocity (V max) and Michaelis-Menten constant (Km) had the values of 0.4 µMole/minutes and 140 m mole/l, respectively. It is conclude that the any subtle alteration in the activity of polyamine oxidase could refer to the intense of damages incurred to the kidney tissue during the kidney failure.

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