Antiviral activity of different misai kucing extracts against herpes simplex virus type 1
Wafaa Abdelwahed Jaheel Alkaby 1 * , Saba Falah 2, Roza Mohamed Hasan 2
1 College of Biotechnology, University of Al-Qadisiyah, IRAQ2 College of Veterinary Medicine, University of Al-Qadisiyah, IRAQ
* Corresponding Author
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 1, pp. 1003-1012
  • Published Online: 05 May 2020
  • Article Views: 176 | Article Download: 129
  • Open Access Full Text (PDF)
AMA 10th edition
In-text citation: (1), (2), (3), etc.
Reference: Jaheel Alkaby WA, Falah S, Hasan RM. Antiviral activity of different misai kucing extracts against herpes simplex virus type 1. Eurasia J Biosci. 2020;14(1), 1003-1012.

APA 6th edition
In-text citation: (Jaheel Alkaby et al., 2020)
Reference: Jaheel Alkaby, W. A., Falah, S., & Hasan, R. M. (2020). Antiviral activity of different misai kucing extracts against herpes simplex virus type 1. Eurasian Journal of Biosciences, 14(1), 1003-1012.

Chicago
In-text citation: (Jaheel Alkaby et al., 2020)
Reference: Jaheel Alkaby, Wafaa Abdelwahed, Saba Falah, and Roza Mohamed Hasan. "Antiviral activity of different misai kucing extracts against herpes simplex virus type 1". Eurasian Journal of Biosciences 2020 14 no. 1 (2020): 1003-1012.

Harvard
In-text citation: (Jaheel Alkaby et al., 2020)
Reference: Jaheel Alkaby, W. A., Falah, S., and Hasan, R. M. (2020). Antiviral activity of different misai kucing extracts against herpes simplex virus type 1. Eurasian Journal of Biosciences, 14(1), pp. 1003-1012.

MLA
In-text citation: (Jaheel Alkaby et al., 2020)
Reference: Jaheel Alkaby, Wafaa Abdelwahed et al. "Antiviral activity of different misai kucing extracts against herpes simplex virus type 1". Eurasian Journal of Biosciences, vol. 14, no. 1, 2020, pp. 1003-1012.

Vancouver
In-text citation: (1), (2), (3), etc.
Reference: Jaheel Alkaby WA, Falah S, Hasan RM. Antiviral activity of different misai kucing extracts against herpes simplex virus type 1. Eurasia J Biosci. 2020;14(1):1003-12.

Abstract

Herpes is a challenging opportunistic infection caused by herpes simplex virus 1 (HSV-1). Emergence of drug resistance is the worst catastrophe that encountered the dedicated efforts to eradicate the disease. This urged the scientists to search for other alternatives from the natural products sanctuary. In this study, aqueous, methanol and ethanol extracts of Orthosiphon stamineus leaves (ALE, MLE & ELE) were prepared and their yield extract and phytochemical contents were determined. Their anti HSV-1 effect was screened using plaque reduction assay and their safety limit was assessed after determining their cytotoxicity against Vero cells using MTT assay. This was followed by mechanism elucidation study which included pre-treatment, attachment, virucidal, penetration, virus yield and time removal and time addition assays. MLE got the highest yield extract and phytochemical content as compared to the others. All of them were considered as non-toxic to Vero cells as their (CC50s > 0.2 mg/ml). Furthermore, their anti-HSV-1 effect shows that all of them had a selective effect in the following order (MLE>ELE>ALE. Their anti-HSV-1 effect was ascribed to their direct virucidal effect or interference with the viral entry through modifying the viral ligand molecules or preventing fusion of the viral envelop with the host cell membrane. In this regard, MLE produced the most powerful effect and it was the only which inhibited the intracellular activity of the virus at all the replication stages. Overall, O. stamineus leaves can be used to develop new antiherpetic agent.

Keywords

Herpes virus Orthosiphon stamineus extract Vero cells and virucidal

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