Antifungal power of chitosan extract from squid pen powder towards Candida albicans
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 2, pp. 3769-3772
  • Published Online: 30 Sep 2020
  • Open Access Full Text (PDF)


Chitosan is a natural material that can be obtained from fishery waste such as crustacean shells and squid pens. Squid pen is a waste produced from the processing of squid with transparent structures with feather-like shapes attached to the dorsal inside a squid mantle. Chitosan from squid pen has been developed in the medical field because it has biocompatible properties, biodegradable, non-toxic, and also has antifungal activity. Being able to eliminate the Candida albicans fungus which is a predisposing factor for the occurrence of denture stomatitis due to the use of long-term dentures. This study aims to determine the antifungal activity of chitosan extracted from squid pen against Candida albicans. Candida albicans was planted in Saboraud broth liquid media tubes and given Chitosan suspension 1.5% (w / v) with each sample group consisting of 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0.78% and there are two control groups namely positive and negative controls. All samples were incubated for 24 hours at 37°C Then a visual observation of turbidity on liquid Saboraud broth media was then carried out by planting culture on SDA media with 7 replications, incubated for 2x24 hours at 37 ° Gmd counted the number of living colonies. The results were analyzed using Kruskal-Wallis and Post hoc tukey test. At the concentration of 25% there was no fungal growth in saboraud dextrose agar media. Chitosan extracted from squid pens has antifungal activity against Candida albicans fungi with a minimum concentration of 25%.


  • Ali, A., Zahid, N., Manickam, S., Siddiqui, Y., Alderson, P. G., & Maqbool, M. (2013). Effectiveness of submicron chitosan dispersions in controlling anthracnose and maintaining quality of dragon fruit. Postharvest Biology and Technology, 86, 147-153.
  • Ariningsih, R. I. (2009). Isolasi Streptomyces dari Rizosfer Familia Poaceae yang Berpotensi Menghasilkan Antijamur Terhadap Candida albicans (Doctoral dissertation, Universitas Muhammadiyah Surakarta).
  • Azrifitria, S. A. (2010). Aktivitas antibakteri ekstrak etanolik daun dan umbi Crinum asiaticum L. terhadap bakteri penyebab jerawat Antibacterial activity of ethanolic extract of leaves and bulbs of Crinum asiaticum L. against acne-inducing bact. Majalah Farmasi Indonesia, 21 (2010).
  • Baskaran, K. (2015). Denture Stomatitis. International Journal of Science and Research. Vol 6, Issue 5. pp. 56-61.
  • Costa, E., Silva, S., Tavaria, F., & Pintado, M. (2014). Antimicrobial and antibiofilm activity of chitosan on the oral pathogen Candida albicans. Pathogens, 3 (4), 908-919.
  • Cuong, H. N., Minh, N. C., Van Hoa, N., & Trung, T. S. (2016). Preparation and characterization of high purity β-chitin from squid pens (Loligo chenisis). International journal of biological macromolecules, 93, 442-447.
  • De Queiroz Antonino, R. S. C. M., Lia Fook, B. R. P., De Oliveira Lima, V. A., De Farias Rached, R. Í., Lima, E. P. N., Da Silva Lima, R. J., ... & Lia Fook, M. V. (2017). Preparation and characterization of chitosan obtained from shells of shrimp (Litopenaeus vannamei Boone). Marine drugs, 15 (5), 141.
  • Goy, R. C., Morais, S. T., & Assis, O. B. (2016). Evaluation of the antimicrobial activity of chitosan and its quaternized derivative on E. coli and S. aureus growth. Revista Brasileira de Farmacognosia, 26 (1), 122-127.
  • Hafdani, F. N., & Sadeghinia, N. (2011). A review on application of chitosan as a natural antimicrobial. World academy of science, engineering and technology, 50, 252-256.
  • Hamzeheinejad, N., & Pal, H. (2016). Mobile Food Consumption Monitoring Application. Journal of Food Technology Research, 3 (2), 88-98.
  • Hemalatha, T., Krithiga, G., Charanya, S., Krishnakumar, S., & Sastry, T. P. (2014). Characterization and evaluation of squid pen derived chitosan scaffold for corneal tissue engineering. Int J Pharm Ther, 5, 76-82.
  • Herawati E & Dwi N. 2017. Denture Stomatitis Terkait Trauma: Gambaran Klinis dan Tatalaksananya. Jurnal Kedokteran Gigi. 29 (4): 22-26.
  • Jeong, S. H., Kim, J. K., Lim, Y. W., Hwang, H. B., Kwon, H. Y., Bae, B. S., & Jin, J. (2018). Squid pen-inspired chitinous functional materials: Hierarchical chitin fibers by centrifugal jet-spinning and transparent chitin fiber-reinforced composite. APL Materials, 6 (1), 016102.
  • Kim, K. M., Son, J. H., Kim, S. K., Weller, C. L., & Hanna, M. A. (2006). Properties of chitosan films as a function of pH and solvent type. Journal of food science, 71 (3), E119-E124.
  • Liang, T. W., Huang, C. T., Dzung, N. A., & Wang, S. L. (2015). Squid pen chitin chitooligomers as food colorants absorbers. Marine drugs, 13 (1), 681-696.
  • Moosazadeh, M., Akbari, M., Tabrizi, R., Ghorbani, A., Golkari, A., Banakar, M., ... & Lankarani, K. B. (2016). Denture stomatitis and Candida Albicans in Iranian population: A systematic review and meta-analysis. Journal of Dentistry, 17 (3 Suppl), 283.
  • Mursida, M., Tasir, T., & Sahriawati, S. (2018). Efektifitas Larutan Alkali pada Proses Deasetilasi dari Berbagai Bahan Baku Kitosan. Jurnal Pengolahan Hasil Perikanan Indonesia, 21 (2), 356-366.
  • Permatasari, D., Budiarti, L. Y., & Apriasari, M. L. (2016). Efektivitas Antifungi Ekstrak Metanol Batang Pisang Mauli (Musa acuminata) dan Chlorhexidine Gluconate 0, 2% terhadap Candida albicans. Dentino, 1 (1), 10-14.
  • Prasanna, S. V., & Lakshmanan, R. (2016). Characteristics, uses and side effects of chlorhexidine-a review. J Dent Med Sci, 15 (6), 57-59.
  • Prashanth, K. H., & Tharanathan, R. N. (2007). Chitin/chitosan: modifications and their unlimited application potential—an overview. Trends in food science & technology, 18 (3), 117-131.
  • Shavandi, A., Bekhit, A. E. D. A., Sun, Z., Ali, A., & Gould, M. (2015). A novel squid pen chitosan/hydroxyapatite/β-tricalcium phosphate composite for bone tissue engineering. Materials Science and Engineering: C, 55, 373-383.
  • Wiyarsih, A. & Priyambodo, E. (2009). Pengaruh Konsentrasi Kitosan dari Cangkang Udang Terhadap Effisiensi Penjerapan Logam Berat (Skripsi). Yogyakarta: Universitas Negeri Yokyakarta. Diakses dari pada tanggal 1 Desember 2018. pp 7.
  • Yien, L., Zin, N. M., Sarwar, A., & Katas, H. (2012). Antifungal activity of chitosan nanoparticles and correlation with their physical properties. International journal of Biomaterials, 2012.


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