Antibacterial screening for five local medicinal plants against nosocomial pathogens: Klebsiella pneumoniae and Staphylococcus epidermidis


Introduction: The continuous increasing in the emergence of antibiotic-resistance in pathogenic bacteria has led to the development of new alternatives that are active against the pathogens. In Iraq, many plant components have been extensive attention as alternative medicine. Materials and Methods: Punica granatum, Mentha pulegium, Allium cepa, Allium sativum and Piper nigrum are five Iraqi medicinal plants were studied in this study. The plant samples were extracted in by three types of solvents, ethanol, methanol and hot boiled water. The plant extracts were examined as antibacterial agents against both Staphylococcus epidermidis and Klebsiella pneumoniae in four concentrations (50, 100, 150, 200 mg/ml) by using the agar-well diffusion method. Results: All assayed plant extracts have antibacterial activity in different concentrations and this activity was varied depending on some factors: the species of bacteria, the type of plant, the type of solvent and the concentration of extract. Depending on the species of bacteria, Gram negative bacteria has higher sensitivity than Gram positive against most studied plant extracts. The type of solvent has a clear effectiveness on the antibacterial activity, the ethanolic extract has the highest antibacterial activity of most studied plants, while the aqueous extract has the lowest activity. On the other hand, the increasing of extract concentration has been associated with increasing of the diameter of inhibition zone. Depending on the type of extracted plant, the maximum diameter of inhibition zone was yielded from Punica granatum extract regardless with the type of the solvent.


  • Al Saimary IE, Bakr SS, Jaffar T, Al-Saimary AE, Salim H, Al-Muosawi R (2002) Effects of some plant extracts and antibiotics on Pseudomonas aeruginosa isolated from various burn cases. Saudi Med. J 23: 802-805.
  • Al-Sa’ady AT, Naher HS (2018) Study of Routs of Etiologic Bacteria Causing Neonatal Infections In Al-Hilla City, Iraq. Biochem. Cell. Arch 18(1): 577-586.
  • AL-Sa’ady AT, Al-Mawla YH (2019) Comparison of Effects Antibiotics and Natural Honey and Extracts of Plants on Escherichia coli Growth Isolated from Different Pathogenic Cases. Journal of University of Babylon for Pure and Applied Sciences 27(3): 420-434.
  • Assefa H, Bogale A, Gebremedhin B, et al. (2016) Village chicken production and marketing in West Gojjam zone, Ethiopia. Current Research in Agricultural Sciences, 3(4): 64-73.
  • Aysa NH (2019) Evaluating of mechanical properties of (silicone/arabic gum/fish hask) composites used as pressure garment prosthetics. Periodicals of Engineering and Natural Sciences 7(3): 1202-1208.
  • Balouiri M, Sadiki M, Ibnsouda SK (2015) Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal 6(2): 71-79.
  • Bassiri-Jahromi S (2018) Punica granatum (Pomegranate) activity in health promotion and cancer prevention. Oncol Rev 12(1): 345. PMCID: PMC5806496.
  • Ceyhan-Güvensen N, Keskin D (2016) Chemical content and antimicrobial properties of three different extracts of Mentha pulegium leaves from Mugla Region, Turkey. J Environ Biol 37(6): 1341-1346.
  • Collee JG, Fraser AG, Marmino BP, Simons A (1996) Mackin and McCartney Practical Medical Microbiology. 14th ed., The Churchill Livingstone, Inc. USA.
  • Debalke D, Mastewal B, Amebaye K, Muluken Y (2018) Assessments of Antibacterial Effects of Aqueous-Ethanolic Extracts of Sida rhombifolia’s Aerial Part. The Scientific World Journal 8.
  • Elisha IL, Francien SB, Lyndy JM, Jacobus NE (2017) The antibacterial activity of extracts of nine plant species with good activity against Escherichia coli against five other bacteria and cytotoxicity of extracts. BMC Complement Altern Med 17: 133. PMCID: PMC5329917.
  • Farag MA, Sara EA, Rashad HH, Hesham RE, Haider NS, Annegret L, Tarek FE, Fouad OFA, Ludger AW (2017) Phytochemical Profiles and Antimicrobial Activities of Allium cepa Red cv. And A. sativum Subjected to Different Drying Methods: A Comparative MS-Based Metabolomics. Molecules 22: 761.
  • Fonkeng LS, Raymond SM, Christopher T, Guy SSN, Monique OK, Nfozon JN, Jules Roger K (2015) Anti Staphylococcus aureus activity of methanol extracts of 12 plants used in Cameroonian folk medicine. BMC Res Notes 24(8): 710.
  • Fuchs AL, Weaver Jr AJ, Tripet BP, Ammons MCB, Teintze M, Copie V (2018) Characterization of the antibacterial activity of Bald’s eyesalve against drug resistant Staphylococcus aureus and Pseudomonas aeruginosa. PloS one 13(11).
  • Hennequin C, Forestier C (2007) Influence of capsule and extended-spectrum beta-lactamases encoding plasmids Upon Klebsiella pneumoniae adhesion Res. Microbiol 158: 339-347.
  • Howell AB, D’Souza DH (2013) The pomegranate: effects on bacteria and viruses that influence human health. Evidence-Based Complementary and Alternative Medicine, 2013.
  • Kadhum SA, Hindi NKK, Al-Musawi S, Alkaim AF (2009) Evaluation of Antimicrobial Activity of the Aquatic Extract against Bacterial isolates from URTI in Babylon Province, Iraq. Journal of Global Pharma Technology 12(9): 296-301.
  • Kyung KH (2012) Antimicrobial properties of Allium species. Curr Opin Biotechnol 23(2): 142-147.
  • Le KY, Dastgheyb S, Ho TV, Otto M (2014) Molecular determinants of staphylococcal biofilm dispersal and structuring. Front. Cell. Infect. Microbiol 4: 167.
  • Lee JH, Park JH, Cho HS, Joo SW, Cho MH, Lee J (2013) Anti-biofilm activities of quercetin and tannic acid against Staphylococcus aureus. Biofouling 29: 491-499.
  • MacFaddin JF (2000) Biochemical tests for identification of medical bacteria. 3rd ed. Williams and Wilkins - Baltimor: 321-400.
  • Mostafa AA, Abdulaziz AA, Khalid SA, Turki MD, Essam NS, Marwah MB (2018) Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases. Saudi Journal of Biological Sciences 25: 361-366. PMCID: PMC5815983.
  • Paterson DL, Ko WC, Von Gottberg A, Mohapatra S, Casellas JM, Goossens H, ... Rice LB (2004) Antibiotic therapy for Klebsiella pneumoniae bacteremia: implications of production of extended-spectrum β-lactamases. Clinical Infectious Diseases 39(1): 31-37.
  • Reiter J, Levina N, Van der Linden M, Gruhlke M, Martin C, Slusarenko AJ (2017) Diallylthiosulfinate (Allicin), a volatile antimicrobial from garlic (Allium sativum), kills human lung pathogenic bacteria, including MDR strains, as a vapor. Molecules 22(10): 1711.
  • Sajjad W, Sohail M, Ali B, Haq A, Din G, Hayat M, Khan I, Ahmad M, Khan S (2015) Antibacterial activity of Punica granatum peel extract. Mycopath 13(2): 105-111.
  • Salehi B, Zainul AZ, Rabin G, Salam AI, Jovana R, Zabta KS, Tariq K, et al. (2019) Piper Species: A Comprehensive Review on Their Phytochemistry, Biological Activities and Applications. Molecules 24(7): 1364.
  • SHAH RH, BALOCH MS, ZUBAIR M, KHAN EA (2017) Phytotoxic Effect of Aqueous Extracts of Different Plant Parts of Milkweed on Weeds and Growth and Yield of Wheat. Planta daninha 35: Viçosa 2017.
  • Sharifi-Rad J, Mnayer D, Tabanelli G, Stojanović-Radić ZZ, Sharifi-Rad M, Yousaf Z, Vallone L, Setzer WN, Iriti M (2016) Plants of the genus Allium as antibacterial agents: From tradition to pharmacy. Cell. Mol. Biol 62(9): 57-68.
  • Vučić V, Grabež M, Trchounian A, Arsić A (2019) Composition and Potential Health Benefits of Pomegranate: A Review. Curr Pharm Des 25(16): 1817-1827.
  • Widerström M, McCullough CA, Coombs GW, Monsen T, Christiansen KJA (2012) multidrug-resistant Staphylococcus epidermidis clone (ST2) is an ongoing cause of hospital-acquired infection in a Western Australian hospital. J Clin. Microbiol 50(6): 2147-51.
  • Yildirim AB, Karakas FP, Turker AU (2013) In vitro antibacterial and antitumor activities of some medicinal plant extracts, growing in Turkey. Asian Pacific Journal of Tropical Medicine 6( 8): 616-624.
  • Zou L, Hu YY, Chen WX (2015) Antibacterial mechanism and activities of black pepper chloroform extract. J Food Sci Technol 52(12): 8196-203.


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