This research was conducted to investigate the potential of using probiotic- experimental based mouthwash on the oral health of canker sores and thrush patients. In accordance, a total of forty children in the age range of one month to ten years old from both sexes were screened orally for lactobacillus isolation during 2019, where a few novel lactobacilli cultures were obtained grown in de Man, Rogosa, Sharpe (MRS) medium. Then after, single lines of lactobacilli cultures were characterized morphologically and biochemically to reveal the isolation of three novel strains of Lactobacillus fermentum with circular, white, glistering, convex colonies. Besides, the Gram staining had indicated, rod-shaped, short, and positive in Gram reaction staining. Besides, the biochemical tests result of the analytical profile index system (API 50 CHL) and Vitek 2 compact had revealed that all the isolates belonged to Lactobacillus fermentum, according to Bergey’s Manual of Systematic Bacteriology. Moreover, the genotypic identification of the isolated strains unveiled the detection of the 1500 bp gene sequence of 16S rRNA for further confirmation. On the other hand, the probiotic activity of the isolated strains were demarcated according to the attributes of the absence of erythrocyte lysis of human and sheep blood in either environment, tolerance to bile salts, growth in different physiological conditions and their antimicrobial activity against Pseudomonas gingivalis and Candida albicans. Furthermore, the potential probiotic activity of the isolated strains was tested for their possible restorative effect combined in probiotic- experimental based mouthwash on the oral health of patients with a canker sore and thrush. Despite the short period for which the patients used the probiotics mouthwash, substantial improvement in the oral and gingival health of patients was observed in the study.

• Ahirwar S S, Gupta M K, Gupta G and Singh V (2017). Screening, isolation and identification of lactobacillus species from dental caries of children. Int J Curr Microbiol App Sci, 6(1), pp.497-503.
• Ambalam P, Ramoliya J, Dave J. and Vyas B (2013). Safety assessment of potential probiotic strains Lactobacillus rhamnosus 231 and Lactobacillus rhamnosus v92 in mouse model. International journal of Bioassays, 2(01),.333-337. www.ijbio.com
• Badet C. and Thebaud N B (2008) Ecology of lactobacilli in the oral cavity: a review of literature. The open microbiology journal, 2,.38. DOI: 10.2174/1874285800802010038
• Balcázar JL, De Blas I, Ruiz-Zarzuela I, Vendrell D, Girones O, and Muzquiz J L (2007) Sequencing of variable regions of the 16S rRNA gene for identification of lactic acid bacteria isolated from the intestinal microbiota of healthy salmonids. Comparative immunology, microbiology and infectious diseases, 30(2), pp.111-118.
• Barzegari A, Kheyrolahzadeh K, Khatibi S M H, Sharifi, S, Memar M Y, and Vahed S Z (2020) The battle of probiotics and their derivatives against biofilms. Infection and Drug Resistance, 13.659. DOI: 10.2147/IDR.S232982
• Blandino G, Fazio D, Petronio G P, Inturri R, Tempera G and Furneri P M (2016). Labeling quality and molecular characterization studies of products containing Lactobacillus spp. strains. International Journal of Immunopathology and Pharmacology, 29(1).121-128. DOI: 10.1177/0394632015600534
• Collado M C, Jalonen L, Meriluoto J, and Salminen S (2006) Protection mechanism of probiotic combination against human pathogens: in vitro adhesion to human intestinal mucus. Asia Pacific Journal of Clinical Nutrition, 15(4).
• Forsythe P. and Bienenstock J, (2010) Immunomodulation by commensal and probiotic bacteria. Immunological investigations, 39(4-5).429-448.
• Gosai V, Ambalam P, Raman M, Kothari C R, Kothari R K, Vyas B R M and Sheth N R, (2011) Protective effect of Lactobacillus rhamnosus 231 against N-Methyl-N′-nitro-N-nitrosoguanidine in animal model. Gut Microbes, 2(6), 319-325.
• Jang S J, Lee K, Kwon B, You HJ and Ko G, (2019) Vaginal lactobacilli inhibit growth and hyphae formation of Candida albicans. Scientific reports, 9(1), 1-9.
• Kelly D, King T and Aminov R (2007) Importance of microbial colonization of the gut in early life to the development of immunity. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 622(1-2), 58-69.
• Khalesi S, Bellissimo N, Vandelanotte C, Williams S, Stanley D and Irwin C, (2019) A review of probiotic supplementation in healthy adults: helpful or hype?. European journal of clinical nutrition, 73(1), pp.24-37.
• Koll P, Mändar R, Marcotte H, Leibur E, Mikelsaar M. and Hammarström L, (2008) Characterization of oral lactobacilli as potential probiotics for oral health. Oral microbiology and immunology, 23(2), pp.139-147.
• Malik V, Tevatia S, Choudhary P, Choudhary, T, Sharma N. and Dodwad V, (2017) Evaluation Of Mouthwash Containing Lactobacillus In Localized Periodontal Pocket As An Adjunct To Non-Surgical Periodontal Therapy In Chronic Periodontitis-A Clinical & Microbiological Study. European Journal of Biomedical, 4(4), 437-441.
• Motisuki C, Lima L M, Spolidorio D M P. and Santos-Pinto L, (2005) Influence of sample type and collection method on Streptococcus mutans and Lactobacillus spp. counts in the oral cavity. Archives of Oral Biology, 50(3), 341-345.
• Piwat S, Teanpaisan R, Thitasomakul S, Thearmontree A. and Dahlen G, (2010) Lactobacillus species and genotypes associated with dental caries in Thai preschool children. Molecular oral microbiology, 25(2), 157-164.
• Reza AT, (2020.) Characterization of Lactobacillus Isolates from Human Mouth and Feces as Probiotics. Cihan University-Erbil Scientific Journal, 4(1), 7-12.
• Rossoni R D, de Barros P P, de Alvarenga J A, Ribeiro F D C, Velloso M D S, Fuchs B B, Mylonakis E, Jorge A O C, and Junqueira J C, (2018) Antifungal activity of clinical Lactobacillus strains against Candida albicans biofilms: identification of potential probiotic candidates to prevent oral Candidiasis. Biofouling, 34(2), 212-225.
• Schrezenmeir J. and de Vrese M, (2001) Probiotics, prebiotics, and synbiotics—approaching a definition. The American journal of clinical nutrition, 73(2), 361s-364s.
• Siqueira W L, de Oliveira E, Mustacchi Z. and Nicolau J, (2004) Electrolyte concentrations in saliva of children aged 6-10 years with Down syndrome. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 98(1), 76-79.
• Songisepp E, Hütt P, Rätsep M, Shkut E, Kõljalg S, Truusalu K, Stsepetova J, Smidt I, Kolk H, Zagura M. and Mikelsaar M (2012) Safety of a probiotic cheese containing Lactobacillus plantarum Tensia according to a variety of health indices in different age groups. Journal of dairy science, 95(10), 5495-5509.
• Tawfeeq A A (2019) Prospective Cohort Study Defining Candida albicans Infections In Some Primary Schools In South of Kirkuk/Iraq 2019. Conference proceedings book:The 6th International Scientific Conference of Genetic and Environment AGERC,14-22.
• Vestman N R, Timby N, Holgerson P L, Kressirer C A, Claesson R, Domellöf M, Öhman C, Tanner A. C, Hernell O. and Johansson I, (2013). Characterization and in vitro properties of oral lactobacilli in breastfed infants. BMC microbiology, 13(1), 193.
• Wall R, Fitzgerald G, Hussey S, Ryan T, Murphy B, Ross P. and Stanton C, (2007) Genomic diversity of cultivable Lactobacillus populations residing in the neonatal and adult gastrointestinal tract. FEMS microbiology ecology, 59(1), 127-137.

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