Alteration of microbial exposure in vitro might aid in child’s relief: docking of isolated oral lactobacillus fermentum with mouthwash as a potential probiotic for children oral health
  • Article Type: Research Article
  • Eurasian Journal of Biosciences, 2020 - Volume 14 Issue 2, pp. 4429-4434
  • Published Online: 26 Oct 2020
  • Open Access Full Text (PDF)

Abstract

This research was conducted to investigate the potential of using probiotic- experimental based mouthwash on the oral health of canker sores and thrush patients. In accordance, a total of forty children in the age range of one month to ten years old from both sexes were screened orally for lactobacillus isolation during 2019, where a few novel lactobacilli cultures were obtained grown in de Man, Rogosa, Sharpe (MRS) medium. Then after, single lines of lactobacilli cultures were characterized morphologically and biochemically to reveal the isolation of three novel strains of Lactobacillus fermentum with circular, white, glistering, convex colonies. Besides, the Gram staining had indicated, rod-shaped, short, and positive in Gram reaction staining. Besides, the biochemical tests result of the analytical profile index system (API 50 CHL) and Vitek 2 compact had revealed that all the isolates belonged to Lactobacillus fermentum, according to Bergey’s Manual of Systematic Bacteriology. Moreover, the genotypic identification of the isolated strains unveiled the detection of the 1500 bp gene sequence of 16S rRNA for further confirmation. On the other hand, the probiotic activity of the isolated strains were demarcated according to the attributes of the absence of erythrocyte lysis of human and sheep blood in either environment, tolerance to bile salts, growth in different physiological conditions and their antimicrobial activity against Pseudomonas gingivalis and Candida albicans. Furthermore, the potential probiotic activity of the isolated strains was tested for their possible restorative effect combined in probiotic- experimental based mouthwash on the oral health of patients with a canker sore and thrush. Despite the short period for which the patients used the probiotics mouthwash, substantial improvement in the oral and gingival health of patients was observed in the study.

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