Based on several active molecules and complex mixture component, bee venom used as folk medicine. We measured the biological effects of bee venom on rat three antioxidant staff in the current study, these were the activity of superoxide dismutase, catalase and malondialdehyde, for which forty rats are being used. The experiment groups were randomly sub-grouped into 8 groups (n=5 for each one). Our results showed no significant variation in superoxide dismutase and catalase activity in all groups whereas Malondialdehyde reveled significant decrease in the bee venom 10 µg/kg after 5 days following injection of CFA in comparison with both control and rheumatoid groups. As conclusion a weak evidence of bee venom effects on three antioxidant under consideration.

• Baskol, G., Demir, H., Baskol, M., Kilic, E., Ates, F., Kocer, D., & Muhtaroglu, S. (2005). Assessment of paraoxonase 1 activity and malondialdehyde levels in patients with rheumatoid arthritis. Clinical biochemistry, 38(10), 951-955.‏
• Fedorova, M., Kuleva, N., & Hoffmann, R. (2009). Reversible and irreversible modifications of skeletal muscle proteins in a rat model of acute oxidative stress. Biochimica et biophysica acta (bba)-molecular basis of disease, 1792(12), 1185-1193.‏
• Frangieh, J., Salma, Y., Haddad, K., Mattei, C., Legros, C., Fajloun, Z., & El Obeid, D. (2019). First characterization of the venom from apis mellifera syriaca, a honeybee from the middle east region. Toxins, 11(4), 191.‏
• Grönwall, C., Amara, K., Hardt, U., Krishnamurthy, A., Steen, J., Engström, M., ... & Greenberg, J. D. (2017). Autoreactivity to malondialdehyde-modifications in rheumatoid arthritis is linked to disease activity and synovial pathogenesis. Journal of autoimmunity, 84, 29-45.‏
• Hadwan, M. H., & Abed, H. N. (2016). Data supporting the spectrophotometric method for the estimation of catalase activity. Data in brief, 6, 194-199.‏
• HARA, A. V., & RATNAVALI, B. www. ijrap. net.‏
• Jazayeri, S., Hoshyarrad, A., Hoseini, F., & Fasihi-Radmandi, M. (2010). Effects of antioxidant supplementations on oxidative stress in rheumatoid arthritis patients. Journal of Biological Sciences, 10(1), 63-66.‏
• Kale, V. M., & Namdeo, A. G. (2014). Antiarthritic effect of galangin isolated from rhizomes of Alpinia officinarum in complete freund’s adjuvantinduced arthritis in rats. Int J Pharm Pharm Sci, 6(4), 499-505.‏
• Kamanlı, A., Nazıroğlu, M., Aydılek, N., & Hacıevlıyagil, C. (2004). Plasma lipid peroxidation and antioxidant levels in patients with rheumatoid arthritis. Cell biochemistry and function, 22(1), 53-57.‏
• Kumar, V., Prakash, J., Gupta, V., & Khan, M. Y. (2016). Antioxidant enzymes in rheumatoid arthritis. J Arthritis, 5(206), 2.‏
• Mahdi, R. K., Al-Hassnawi, A. T. S., & AL-Rubaei, H. M. (2019, August). Biochemical investigation effect of Vespa orientalis venom therapy: Rat rheumatoid arthritis model. In AIP Conference Proceedings (Vol. 2144, No. 1, p. 040004). AIP Publishing LLC.‏
• Marklund, S., & Marklund, G. (1974). Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European journal of biochemistry, 47(3), 469-474.‏
• Mokosuli, Y. S., Repi, R. A., & Worang, R. L. (2017). Potential antioxidant and anticancer effect of Apis dorsata Binghami Crude Venom from Minahasa, North Sulawesi.‏
• Nathan, C., & Cunningham-Bussel, A. (2013). Beyond oxidative stress: an immunologist’s guide to reactive oxygen species. Nature Reviews Immunology, 13(5), 349-361.‏
• Nunes-Miranda, J. D., Bancel, E., Viala, D., Chambon, C., Capelo, J. L., Branlard, G., ... & Igrejas, G. (2017). Wheat glutenin: the “tail” of the 1By protein subunits. Journal of proteomics, 169, 136-142.‏
• Oghenesuvwe, E. E., & Paul, C. (2019). Edible insects bio-actives as anti-oxidants: Current status and perspectives. JOURNAL OF COMPLEMENTARY MEDICINE, 10(2), 89-102.‏
• Orhevba, B., Adebayo, S. E., & Salihu, A. (2016). Synthesis of Biodiesel from Tropical Almond (Terminalia Catappa) Seed Oil. Current Research in Agricultural Sciences, 3(4), 57-63.
• Quiñonez-Flores, C. M., González-Chávez, S. A., Del Río Nájera, D., & Pacheco-Tena, C. (2016). Oxidative stress relevance in the pathogenesis of the rheumatoid arthritis: a systematic review. BioMed research international, 2016.‏
• Quiñonez-Flores, C. M., González-Chávez, S. A., Del Río Nájera, D., & Pacheco-Tena, C. (2016). Oxidative stress relevance in the pathogenesis of the rheumatoid arthritis: a systematic review. BioMed research international, 2016.‏
• Sarban, S., Kocyigit, A., Yazar, M., & Isikan, U. E. (2005). Plasma total antioxidant capacity, lipid peroxidation, and erythrocyte antioxidant enzyme activities in patients with rheumatoid arthritis and osteoarthritis. Clinical biochemistry, 38(11), 981-986.‏
• Souza, J. M., Peluffo, G., & Radi, R. (2008). Protein tyrosine nitration—functional alteration or just a biomarker?. Free Radical Biology and Medicine, 45(4), 357-366.‏
• White, B. C., Krause, G. S., Aust, S. D., & Eyster, G. E. (1985). Postischemic tissue injury by iron-mediated free radical lipid peroxidation. Annals of emergency medicine, 14(8), 804-809.‏
• Yamada, T., Abe, M., Lee, J., Tatebayashi, D., Himori, K., Kanzaki, K., ... & Lanner, J. T. (2015). Muscle dysfunction associated with adjuvant-induced arthritis is prevented by antioxidant treatment. Skeletal muscle, 5(1), 1-10.‏
• Yamada, T., Fedotovskaya, O., Cheng, A. J., Cornachione, A. S., Minozzo, F. C., Aulin, C., ... & Lundberg, I. E. (2015). Nitrosative modifications of the Ca2+ release complex and actin underlie arthritis-induced muscle weakness. Annals of the rheumatic diseases, 74(10), 1907-1914.‏
• Yamada, T., Steinz, M. M., Kenne, E., & Lanner, J. T. (2017). Muscle weakness in rheumatoid arthritis: the role of Ca2+ and free radical signaling. EBioMedicine, 23, 12-19.‏

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.