A study to detect the most important virulence factors of cryptosporidium parasite samples by PCR

Abstract

Cryptosporidium parvum is a coccidian parasite causing diarrhea. The aim of our study is the detection of the infective species with virulence factors in Cryptosporidium in children who have diarrhea in Diwaniyah city. The total fecal samples were one hundred; take from diarrheic children who are aged below 10 years old in Maternity and Childhood Teaching Hospital. Oocytes of Cryptosporidium oocytes were 29 (29%) in children with diarrhea using a light microscope with an acid-fast stain for the samples. Findings of polymerase chain reaction showed that C. parvum is an accusative agent of the cryptosporidiosis in children and all these samples contain the Glycoprotein 900 pathogenic factor under study.

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