In West Bengal, India, Desmostachya bipinnata L. Stapf (Poaceae), Parthenium hysterophorus L. Asteraceae and Alternanthera sessilis L. R. Br. ex DC. (Amaranthaceae), are three weeds with potential allelochemicals. An investigation therefore was carried out for the determination and evaluation of their allelopathic potential in terms of relative allelopathic potential. Allelochemicals in various concentrations [1:5, 1:10 and 1:20 (w/v)] of leaf extracts and leachates of the three weeds respectively were tested on Allium cepa root tips which is generally a very responsive bioassay material for sensing allelopathic property. Cytological evaluation of allelopathic potential is done by microscopic study of allelochemical induced alterations of cytological characters with particular reference to various anomalies during cell-division and chromosomal structural abnormalities like condensation, pole deviation, anaphase bridge, clumping, bi-nucleation, depurination, fragmentation, stickiness, multinucleate cells, late separation with micronuclei formation. Chromosomal Abnormality Index reveal that Desmostachya bipinnata> Parthenium hysterophorus > Alternanthera sessilis in terms of allelopathic potential inducing chromosomal abnormalities in Allium cepa root tips indicating that the monocotyledonous weed Desmostachya is more allelopathically vigorous than the other two dicotyledonous weeds- Parthenium and Alternanthera. Further research is thus required to know the interaction of other invasive dicotyledonous and monocotyledonous weeds on other weed and crop species giving newer dimensions to allelopathy research.


  • Andrioli NB, Soloneski S, Larramendy ML, Mudry MD (2012) Cytogenetic and microtubule array effects of the zineb-containing commercial fungicide formulation Azzurro® on meristematic root cells of Allium cepa L. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 742(1-2): 48-53.
  • Herrero O, Martín JP, Freire PF, López LC, Peropadre A, Hazen MJ (2012) Toxicological evaluation of three contaminants of emerging concern by use of the Allium cepa test. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 743(1-2): 20-24.
  • Iannacone JO, Onofre RC, Huanqui OS, Giraldo JA, Mamani NP, Miglio MC, Alvariño LF (2007) Environmental risk assessment of the insecticide metamidophos in bioassays with four non-target aquatic organisms. Agricultura Tecnica, 67(2): 126-138.
  • Inderjit, Dakshini KMM (1998) Allelopathic interference of chickweed, Stellaria media with seeding growth of wheat (Triticum aestivum). Canadian Journal of Botany 76: 1317-1321.
  • Inderjit, Weiner J (2001) Plant allelochemical interference or soil chemical ecology? Perspectives in Plant Ecology Evolution and Systematics 4(1): 3-12. https://doi.org/10.1078/1433-8319-00011
  • Leme DM, Marin-Morales MA (2009) Allium cepa test in environmental monitoring: a review on its application. Mutation Research/Reviews in Mutation Research, 682(1): 71-81.
  • Marimuthu KM, Subramanium K (1960) A haematoxylin squash method for the root tips of Dolichos lablab Linn. Curr. Sci., 29: 482-483.
  • Reinhardt P, Causa M, Marian CM, Hess BA (1996) Physical Review, B54: 14812-14821.
  • Panse VG, Sukhatme PV (1967) Statistical methods for agricultural workers. 2nd edition, pp. 150-157. Indian Council of Agricultural Research, New Delhi, India.
  • Rice EL (1984) Allelopathy pp. 424. 2nd edition. New York, Academic Press. Orlando. FL.
  • Rizvi SJH, Rizvi V (1992) Allelopathy: basic and applied aspects. Chapman and Hall, London: pp. 480.
  • Ohno T (2001) Journal of Environmental Quality 30: 1631-1635.
  • Wittmann W (1962) Aceto-iron-haematoxylin for staining chromosomes in squashes of plant material. Stain technology, 37(1): 27-30.
  • Simelane MM, Masuku MB, Rugambisa JI, Earnshaw DM (2016) Analysis of Consumer Willingness to Pay for Genetically Modified Maize Food in Swaziland. International Journal of Sustainable Agricultural Research, 3(1): 19-28.


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