EurAsian Journal of BioSciences (Eurasia J Biosci, e-ISSN 1307-9867) is an international, refereed electronic journal. It publishes the results of original research in the field of biological sciences especially related to morphology, physiology, genetics, ethnobiology, ethnobotany, taxonomy, ecology and biogeography of both prokaryotic and eukaryotic organisms.

Advantages of publishing in Eurasia J Biosci

  • Independent, rigorous peer review
  • Rapid publication
  • Open access – gives a worldwide audience larger than that of any subscription-based journal and thus increases the visibility and impact of published works. 
  • Quality: Eurasia J Biosci applies the professional copyediting and typesetting processes.
  • Indexed in major databasesEurasia J Biosci is indexed by AGRICOLA, EBSCOhost, ProQuest, SCOPUS, SCImago, Zoological Record (Thomson Reuters) etc. All published articles are assigned to Digital Object Identifier (DOI)- CrossRef. 

Current Issue (Volume 13, Issue 2, August-December 2019)

(In Progress)

Determination of environmental considerations for the use of dispersant species in different areas of the Caspian Sea
Alaleh Gholipour Peyvandi

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Abstract

The diversification of activities on the shores on the one hand and the conflict between operators or part programs on the other hand make the monitoring and optimal management of resources in the coastal areas of the country compulsory.
An example of coastal issues that requires continuous and integrated management can be mentioned in some cases, such as coastal environmental issues and preventing possible damage to beaches. Addressing or reducing these problems is a serious challenge for local governments and central governments that, if continued, their harmful effects could have irreparable effects and damages. Therefore, beaches should be managed in such a way that they can be exploited. Rationalize the resources and capacities available.
One of the important issues related to coastal waters and their pollution is pollution of oil, and it is presented in order to reduce this important and effective pollutant which one of them is chemical purification, or the use of dispersant oil spots (dispersants). In this research, discussion of various types of dispersants has been carried out, and tests for toxicity and efficacy testing as well as quantitative testing of these materials have been carried out.
In the efficacy study it was found that if dispersant is effective, a quaternary solution is created which causes the water to become cloudy, and the reference solution that is used is not transparent, and only the oil spots are visible inside the water column. In the quantitative determination test 4 standard mixtures which each represent the performance of these materials, namely 100% efficiency, 75%, 50% and 25%.
In the quantification test, four standard mixtures prepared, that each represent the performance of these materials, namely 100%, 75%, 50%, and 25%.
In addition, the treatment of oil with dispersants, due to increased concentration of dissolved oil in water, increases the effect of direct oil toxicity on plant and animal life. Also, the relationship between the efficacy of dispersant and the three viscosity, temperature and flow factors was investigated and it was concluded that the effectiveness of dispersant decreased with increasing viscosity; Some dispersants in higher temperatures and with lower salinity and other low temperature dispersants operate with higher salinity.

Keywords: dispersant, oil pollution, Lc50, toxicity, viscosity

Citation: Peyvandi AG. Determination of environmental considerations for the use of dispersant species in different areas of the Caspian Sea. Eurasia J Biosci. 2019;13(2):651-62.

Analysis of the current situation related to the food security of indigenous population of the Northern Russia
I. R. Shegelman, P. O. Shchukin, A. S. Vasilev

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Abstract

The food security of indigenous population of the Russian Northern regions represents quite a pressing problem and there are a number of important factors that should be considered when studying the issue. The composition of food products included in the diet of indigenous population of different countries and Russia, the methods of food preparation and preservation during centuries were influenced by the natural and productive conditions of life, work and leisure, which necessitates the study of specific nature of such nutrition. Over the last years, the diet culture of the indigenous population of the Northern Russia changed significantly influenced by a number of reasons: the industrialization of the regions and the organized development of the enormous raw material resources located there (minerals, forest resources etc.); the impact of migration processes, which led to the broad northern areas of Russia being inhabited by the population from the center and south of Russia, as well as from other territories of the Soviet Union (which is particularly evident in the late 1940s and subsequent post-war years) that had own diet culture; organized access of the population to a variety of store-bought food products, which significantly replaced the traditional food. The article provides the analysis of the current aspects of food security of indigenous population of the Northern Russia aimed at identification of nutrition patterns of different ethnic groups of the Northern Russia, promoting the population’s efficient involvement in life activities.

Keywords: indigenous population, food security, Northern Russia, nutrition, traditional diet

Citation: Shegelman IR, Shchukin PO, Vasilev AS. Analysis of the current situation related to the food security of indigenous population of the Northern Russia. Eurasia J Biosci. 2019;13(2):663-72.

Growth of sesame (Sesamum indicum L.) plants with mediated compost biochar on coastal sandy land area in Bantul Regency Indonesia
Dewi Ratna Nurhayati, Siswadi

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Abstract

This study aims to determine the growth of sesame plants that applied biochar organic matter on coastal sandy land. The coastal sandy land area of Indonesia is large enough. It is known as one of the marginal lands that have low productivity due to dominant soil constituent material of sand (>80%) so that it affects the availability of water and plant nutrient negatively. To improve the water-holding capacity, an applied technology is urgently needed so that it can be used as a growing material of sesame. A novel technology through the use of specific biochar, activated coconut shell charcoal, was proposed. Due to its functions in optimizing growing medium, improving soil properties physically, chemically, and biologically as well as in holding water and providing nutrients, the used biological charcoal would work as biological soil amendments. It is expected that biochar can be continuous sources for plant needs. The experiment was factorial design laid out in Randomized Complete Block Design involved 14 treatments with three replications consisting of combinations of seven charcoal applications and two sesame varieties. The data were subjected to analysis of variance and Tukey’s Honestly Significant Difference test were used as a post-hoc analysis (p<0.05). The experimental results showed that the optimum plant growth was obtained from the application of coconut shell charcoal at a dose of 10 ton/ha combined with chicken manure at a dose of 30 ton/ha, such as tallest in height, highest net assimilation rate, and had most flowers.

Keywords: growth sesame plant, biochar, biochar on sand land, Coastal Sandy Land

Citation: Nurhayati DR, Siswadi. Growth of sesame (Sesamum indicum L.) plants with mediated compost biochar on coastal sandy land area in Bantul Regency Indonesia. Eurasia J Biosci. 2019;13(2):673-9.

Evaluation of canola germination characteristics under priming condition
Nasrollah Atashi Shirazi, Foroud Bazrafshan, Omid Alizadeh, Koroush Ordookhani, Alireza Safahani Langroodi

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Abstract

This study performed to evaluation of priming and priming duration effects on some germination’ characteristics of two canola cultivars (Zurika and Talayeh) as factorial randomized complete design with three replications at the Besat Research Center, Fars Province, Iran. The first factor treatments included cultivars and second factor was salicylic acid in 3 levels (750, 1500 and 2250 M), ascorbic acid in 3 levels (200, 400 and 600 ppm), polyethylene glycol on 2 levels (-2 and -4 bar), potassium chloride 2 level (-5 and -10 bar) Brassinosteroid on 2 levels (20 and 40 micrograms per liter), gibberellic acid on 2 levels (100 and 200 mg), nano zinc chelate and nitrogen each at two levels (1 and 2 mg) and priming water, also third factor included priming duration at 3 levels (3, 6 and 12 h). Totally, according to result it was founded that priming had positive effect on germination characteristics also it was determined that gibberellic acid, salicylic acid and Ascorbic acid had highest effects in compared to other priming, with increasing of concentration and duration of priming, studied traits increased in responses to treatments. According to interaction between priming and duration, it was founding that highest radicle length (6.13 mm) and plumule weight (0.172 g) were obtained by BR20 for 3h duration, also KCL-5 treatment with 12h duration showed highest means for plumule length (4.19mm) and seedling length (52.37mm). The results showed that highest seedling weight (0.315 g), allometric coefficient (1.074) and weight vigor index (0.218) were obtained by GA100 for 3h.

Keywords: canola, cultivar, priming

Citation: Shirazi NA, Bazrafshan F, Alizadeh O, Ordookhani K, Langroodi AS. Evaluation of canola germination characteristics under priming condition. Eurasia J Biosci. 2019;13(2):681-6.

Investigation of the influence of the thermal effects on the FeCrAl alloys Kh15Yu5 and Kh23Yu5
B. Kh. Khusain, A. R. Brodskiy, V. I. Yaskevich, M. Zh. Zhurinov, A. Z. Abilmagzhanov

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Abstract

FeCrAl alloys having the composition of chromium added to iron is peculiar by in the increased corrosion resistance of the new metallic alloy compared to pure iron. Such FeChAl alloys are used in numerous different applications as a corrosion resistant, oxidation resistant and heat resistant material. Owing to their resistance and good mechanical characteristics, FeCrAl alloys are regarded as one of the main structural materials for use in nuclear power plants. They are commonly used within industrial applications where high-temperature oxidation resistance is needed. The good resistance to oxidation, sulfur resistance and corrosion resistance are attained due to a dense oxide film formed on the surface that prevents the rapid further degradation of the material. In this study, two FeCrAl alloys are investigated - Kh15Yu5 (15%Cr5%Al) and Kh23Yu5 (23%Cr5%Al). Using the method of Mössbauer spectroscopy, the study was conducted on the influence of temperature and period of heating on the cold-rolled ribbon of FeCrAl alloys Kh15Yu5 and Kh23Yu5 with the thickness of 50 µm. It is shown that these alloys, as a result of thermal effect, are prone to the layering in solid solution, forming the areas depleted and enriched with chromium and aluminum.

Keywords: FeCrAl alloys, chromium-containing alloys, Mössbauer spectroscopy, effective magnetic field, impurity atoms, solid solution layering

Citation: Khusain BK, Brodskiy AR, Yaskevich VI, Zhurinov MZ, Abilmagzhanov AZ. Investigation of the influence of the thermal effects on the FeCrAl alloys Kh15Yu5 and Kh23Yu5. Eurasia J Biosci. 2019;13(2):687-94.

Assessing of the learning needs of nurses in medical and surgical and emergency wards: nursing continuing education requirements
Shamaneh Mohamadi, Fariba Borhani, Malahat Nikravan-Mofrad, Abbas Abbaszadeh, Farshid Monajemi, Hossein Roohi Moghaddam

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Abstract

Introduction: Continuing education (CE) is increasingly needed for nurses to keep pace with the rapid changes in patient care. A Learning Need Assessment (LNA) is a systematic approach to examining what individuals or groups need to learn. LNAs are often associated primarily with continuing education. Current CE programs are not well designed for showing nurses’ learning needs. Few programs are underpinned by needs analysis in some countries.
Design and Method: This cross-sectional study was conducted to assess the learning needs of clinically based nurses within medical, surgical and emergency units in Tehran (capital of Iran) hospitals. Data were collected using a learning needs assessment questionnaire developed based on nursing texts and literatures with calculated CVI equal to 0.91 and Chronbach Alpha equal to 0.87. It includes demographic information and 30 learning need for nurses. Nurses that had adequate experience in clinical nursing conveniently selected and determined their learning need.
Results: Ten educational need priorities were determined with 314 nurses, included: tele-nursing (distance-care) (71.60%), hospital infection control and personal protection (61.80%), new approaches of patient education (58.7%), professional communication (57.4%), safety and risk management (53.4%), wound nursing care and New way dressing (50.6%), Nursing research (49.5%), Nursing Law (48.1%), Nursing management (46.9%), Correct Reporting and documentation (46.3%) were among the top 10 education priorities, and over 45% of nurses needed full education.
Conclusion: Learning need assessment is an important and first step of educational planning for continuing education that can provide useful, effective and cost effective knowledge and skills for nurses.

Keywords: learning need assessment, continuing education, medical and surgical nurses, nursing education

Citation: Mohamadi S, Borhani F, Nikravan-Mofrad M, Abbaszadeh A, Monajemi F, Moghaddam HR. Assessing of the learning needs of nurses in medical and surgical and emergency wards: nursing continuing education requirements. Eurasia J Biosci. 2019;13(2):695-700.

SOCS (Suppressor of Cytokine Signaling) protein as material to enhance the effect of growth hormone in broilers
Anwar Ma’ruf, Nunuk Dyah Retno L, Ratna Damayanti, Nove Hidajati, M. Gandul Atik

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Abstract

The purpose of this study was to identify specific proteins that can slow down the SOCS protein activity in STAT signaling protein termination so that it will induce the increase in GH metabolic effect in enhancing the growth of broilers and their meat quality. It is, therefore, necessary to identify the molecular weight and the composition of the SOCS (Suppressor of Cytokine Signaling) protein acid that act in the signaling of STAT (Signal Transducers and Activators of Transcription) activated by GH (Growth Hormone) in broilers. By identifying the molecular weight and the composition of the amino acids of SOCS proteins, there is a great chance to make a specific protein that can slow down the SOCS protein activity in STAT signaling protein termination. Therefore, there is an increase in GH metabolic effect in enhancing the broilers’ growth and their meat quality. The results indicated that SOCS-1 protein was present in broilers’ liver tissue. An examination with Western Blot noted that the molecular weight of the SOCS-1 protein was 98 kDa. This finding signifies that SOCS-1 protein is a protein that serves as negative feedback on broiler growth through growth hormone.

Keywords: protein SOCS, growth hormone, growth, broiler

Citation: Ma’ruf A, Retno L ND, Damayanti R, Hidajati N, Atik MG. SOCS (Suppressor of Cytokine Signaling) protein as material to enhance the effect of growth hormone in broilers. Eurasia J Biosci. 2019;13(2):701-5.

Muskrat’s (Ondatra Zibetica) endicrine regulation organs as bioindicators for evaluation of ecological conditions in Baikal Region
I. F. Zolnikova, I. I. Silkin, A. P. Popov, E. A. Tomitova, N. D. Ovcharenko

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Abstract

The article describes the morphometric measurements of the adrenal cortex functional zones and analyzes the results of these measurements and the percentage correlation of the zones to the total adrenal cortex area. Besides, an analysis of the functional activity of the adrenal cortex zona fasciculate is presented. The research has shown that the highest tension of the adrenal gland function occurs in the animals inhabiting the north-west outskirts of Irkutsk (Novo-Lenino district). They have a thicker adrenal cortex zona fasciculate and reticular zone as well as a higher functional activity of the reticular zone in comparison to other study groups. This is probably indicative of an intensive production of glykocorticoid, androgens, and estrogens. On these grounds we can assume that this district is the most hazardous for the existence of living organisms which we believe to be a sign of unfavorable ecological conditions. The use of the muskrat as a bioindicator for the evaluation of the ecological conditions in different city districts and the Selenga River delta is unprecedented.

Keywords: muskrat, test object, bioindicator, adrenal cortex, nuclear-cytoplasmic ratio

Citation: Zolnikova IF, Silkin II, Popov AP, Tomitova EA, Ovcharenko ND. Muskrat’s (Ondatra Zibetica) endicrine regulation organs as bioindicators for evaluation of ecological conditions in Baikal Region. Eurasia J Biosci. 2019;13(2):707-9.

Evaluation of auxin priming and plant growth promoting Rhizobacteria on yield and yield components of wheat under drought stress
Najmeh Bagheri, Omid Alizadeh, Shahram Sharaf Zadeh, Farshid Aref, Kourosh Ordookhani

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Abstract

This study was performed to the evaluation of auxin and plant growth promoting rhizobacteria priming on yield and yield components of wheat under drought stress. Experiment was conducted as a Split Split plot in RCBD design with 3 replications. Main factor included drought stress (irrigation on base of 25, 50 and 75% FC) and sub plots were inoculation at 4 levels (control, azotobacter, pseudomonas and a combination of two bacteria), also sub sub plot were auxin priming at 4 levels (0, 1, 2 and 4 mg.L-1). According to results, application of drought stress led to the reduction grain yield, so, the 50% and 75% water discharge treatments led to 9% and 37% the reduction of grain yield as compared to control, respectively. In relation to bacteria treatments, highest grain yield (4.35 t/ha) obtained by azotobacter inoculation. In relation to auxin application, the 4 mg. L-1 treatment led to 6% the increase of grain yield as compared to control. Highest means of grain yield (3.25 t/ha) under 75% discharge water observed by azotobacter inoculation. We concluded that priming with auxin and rhizobacteria, plays an important role in the induction of tolerance to drought and overcome limitations created by the drought stress on wheat.

Keywords: bacteria, plant regulator, priming, yield, wheat

Citation: Bagheri N, Alizadeh O, Sharaf Zadeh S, Aref F, Ordookhani K. Evaluation of auxin priming and plant growth promoting Rhizobacteria on yield and yield components of wheat under drought stress. Eurasia J Biosci. 2019;13(2):711-6.

Seasonal development of introduced apple-tree varieties under arid conditions of Western Kazakhstan
O. N. Kosareva, G. E. Dinova

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Abstract

For the first time the article presents perennial statistically processed results of pheno-observations for 11 apple varieties introduced into the Mangyshlaksky Experimental Botanical Garden, located in the west of Kazakhstan, whose natural conditions are characterized by sharp fluctuations of air and soil temperature in both summer and winter periods, aridity and high content of readily soluble salts in soil with underlying slab of Sarmatian limestone. They determined the average phenodates of the main phenophases and the average duration of growth and development periods among apple varieties, and their variability was revealed. It was noted that generative organs are characterized by significantly lower variability than vegetative ones. In local conditions, there was a significant decrease in the fruiting period, the decrease of yield and the average weight of fruits. The most resistant and productive varieties were selected. The work was conducted on the grant project topic “Introduction of apple tree promising varieties into the culture in arid regions of Western Kazakhstan”.

Keywords: apple tree, varieties, introduction, arid conditions, salinization, phenodates, phenophases, growth and development rhythms, the growth of shoots, yield, fruit weight, variability, resistance

Citation: Kosareva ON, Dinova GE. Seasonal development of introduced apple-tree varieties under arid conditions of Western Kazakhstan. Eurasia J Biosci. 2019;13(2):717-27.

The composition of the amino acids protein ghrelin and neuropeptide y as the basis for regulating energy balance in broilers
Nove Hidajati, Ratna Damayanti, Anwar Ma’ruf

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Abstract

The purpose of this study was to determine the molecular weight of the protein ghrelin as a basis to determine the amino acid composition of protein ghrelin and subsequently to make synthetic ghrelin protein whose function is to control energy balance in broilers. Samples isolated from the digestive tract and brain tissue of the broilers and then examined by SDS Page and the Western blot test. Based on the results, it can be concluded that the protein ghrelin had a molecular weight of 44 kDa and composed of methionine, phenylalanine, leucine, arginine, valine, isoleucine, and leucine.

Keywords: ghrelin, energy, feed efficiency, broiler

Citation: Hidajati N, Damayanti R, Ma’ruf A. The composition of the amino acids protein ghrelin and neuropeptide y as the basis for regulating energy balance in broilers. Eurasia J Biosci. 2019;13(2):729-32.

Features of intra population variability of Medicago varia Mart. with the expressed mf-mutation on a complex qualitative characteristics
Vladimir I. Cherniavskih, Elena V. Dumacheva, Zhanna A. Borodaeva, Anzhelika A. Gorbacheva, Elena N. Horolskaya, Larisa Ch. Gagieva, Nadezhda V. Kotsareva, Svetlana V. Korolkova

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Abstract

Lucerne has multifunctional value: it is a source of a high-protein forage for farm animals and valuable raw materials for pharmaceutical industry. The purpose of this work was studying of features of intra population variability of a lucerne with a mf-mutation on a complex of the qualitative signs, valuable as for the forage production as well as for the pharmaceutical industry. In the years 2016-2018, researchers of the 30 breed population, received as a result of an individual selections from a breed lucerne Krasnoyaruzhskaya 1 on the basis of a high expression of a mutation of multifoliolation (mf-mutation). The high variability of a population of the lucerne in the posterity of the second generation on the basis of expressiveness of the mf-mutation was established. The breed population was distributed by the index of an expression of the mf-mutation as follows: the group with low expressiveness of the sign included 52.8% of numbers, 9.9% of the numbers had the index of an expression up to the standard, 36.3% of the breed population exceeded the standard for 9.3-62.7%. As a result of researches the direct correlation between degree of an expression of the mf-mutation and the feeding efficiency of a lucerne is not revealed. There was established the breed population SP-11 at which the high expression of the mf-mutation and high efficiency of the elevated phytoweight against the background of the low maintenance of saponin and antotcyanin. The population of SP-11 will be included in the selection program for the creation of the lucerne breeds with a high fodder quality. The breed population SP-23, SP-24, SP-28 has a high expression of the mf-mutation, high efficiency of the elevated phytoweight against the background of the high content of the biologically active agents (saponins and antocyanins). These forms will be included in the selection program for creation of breeds of a lucerne with the high content of biologically active agents for the pharmaceutical industry.

Keywords: Medicago varia Mart., multifoliolation, mf-mutation, saponins, antocyanins, feeding efficiency, raw materials for the pharmaceutical industry

Citation: Cherniavskih VI, Dumacheva EV, Borodaeva ZA, Gorbacheva AA, Horolskaya EN, Gagieva LC, et al. Features of intra population variability of Medicago varia Mart. with the expressed mf-mutation on a complex qualitative characteristics. Eurasia J Biosci. 2019;13(2):733-7.

Bioremidation of heavy metals and role of bacteria
Darya Fazaeli

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Abstract

Background and Objective: Contamination of aquatic and soil ecosystems by heavy metals has been always a threat for environment and health of human communities. Bioremidation is a new method that can remove this problem. This study aimed to examine removal of heavy metal by bacteria.
Method: This review study was conducted on studied about bioremidation of heavy metals with bacteria. The applied sites included Sid.ir-Science direct-ncbi-.ncbi.civilica. Inclusion criterion was having relevant information and keywords.
Findings: The methods used in bioremidation of heavy metals are more efficient related to physic-chemical methods. Bacteria can reduce concentration and toxicity of heavy metals in different ways due to their various resistance mechanisms compared with heavy metals.
Conclusion: The results obtained from this study showed that bioremidation is an efficient and proper method to remove heavy metals pollution and bacteria can be used for bioremidation of heavy metals from the environment.

Keywords: heavy metals, bioremidation, bacteria, biosorption

Citation: Fazaeli D. Bioremidation of heavy metals and role of bacteria. Eurasia J Biosci. 2019;13(2):739-44.

The traditional values of Topat War for spring conservation at Lingsar, West Lombok-West Nusa Tenggara Indonesia
Ahmad Jupri, Zaenal Kusuma

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Abstract

The celebration of local wisdom conducted is filled of many ideas and values to manage and conservation of natural resources including water resources. This research aims to investigate relationship between utilization and management of natural resources with Topat war as local wisdom. The study was conducted at Lingsar village, West Lombok Regency, Indonesia. Qualitative research method was used to explore the social, economic and culture of Lombok community. Data were collected from respondent which were determined as community leaders, traditional leaders and ordinary community member. The result show that the traditional values in Topat war can be applied in the management of water resources. The residents of Lingsar use the water wisely either for agriculture, fisheries or livestock purposes. Lingsar community is still firmly hold strong and prominent value of mutual cooperation, social relations, mutual respect, tolerance, family tolerance and mutual living of social status, economic status between each other.

Keywords: local wisdom, spring conservation, water management, water resource

Citation: Jupri A, Kusuma Z. The traditional values of Topat War for spring conservation at Lingsar, West Lombok-West Nusa Tenggara Indonesia. Eurasia J Biosci. 2019;13(2):745-8.

Mechanization of the haylage rolls wrapped with film on the basis of the modernization of drive of the mower-conditioner cutting machine
Omirserik Zhortuylov, Anuarbek Adilsheyev, Askar Rzaliyev, Gani Zhumatay, Ulan Bekenov, Azamat Zhortuylov

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Abstract

Harvesting haylage in rolls is one of the most effective ways of harvesting grass fodder. High-quality haylage cannot be obtained without the use of mowers-conditioners, reducing the drying time of the grass mass. The method of vector contours was used to study the crank-beam-drive mechanism of the mower-conditioner knife drive. Analytical equations are obtained, which describe the laws of travel, speed and acceleration of the knife movement. The parameters and modes of the knife drive mechanism are substantiated, tested under production conditions, which allow to reduce the power required by 1.6 times and partially reduce the oscillations of the knife.

Keywords: haylage, roll, technology, winding, film, crank-beam mechanism, drive, cutting unit, inertial forces, crank, double knife stroke

Citation: Zhortuylov O, Adilsheyev A, Rzaliyev A, Zhumatay G, Bekenov U, Zhortuylov A. Mechanization of the haylage rolls wrapped with film on the basis of the modernization of drive of the mower-conditioner cutting machine. Eurasia J Biosci. 2019;13(2):749-56.

The antidiabetic effect of bitter melon (Momordica charantia L.) extracts towards glucose concentration, langerhans islets, and leydig cells of hyperglycemic mice (Rattus norvegicus)
Dewa Ketut Meles, Wurlina, Dewa Putu Anom Adnyana, Chaterina Puspadewanti Rinaldhi, Rian Rizky Octaviani, Desak Ketut Sekar Cempaka

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Abstract

Purpose: This study was aimed to prove the fruit extract of bitter melon (Momordica charantia L.) towards blood sugar levels, of Langerhans islets cells, and Leydig cells of hyperglycemic white mice (Rattus norvegicus).
Methods: Twenty-five mice were divided randomly into 5 groups. They all were induced intraperitoneally by alloxan with dosage of 150 mg/kg in order to damage to the pancreas. From all treatments group, three groups (P1, P2, and P3) were treated with various doses of bitter melon extract with dosage 29, 50, and 59 mg/1 ml/day, respectively. As a comparative group, –negative control group (P0 +) were given with CMC-Na 0,5% 1ml/day, whereas the positive control group (K+) were given Glibenclamide® 0.126 mg/1 ml/day. Bitter melon extract was given for 21 days. In the first day of treatment, blood glucose level of mice was examined after 2 hours, 4 hours, 6 hours, 8 hours after treatments. The blood glucose examinations were subsequently continued at days 7th, 14th and 21st after treatment. After 21 days, the pancreas and testes of mice were taken for histopathological preparations made.
Results: Bitter melon (Momordica charantia L.) extract had antidiabetic effects that can lower blood glucose level, improved pancreatic beta cell damage, and increased the Leydig cells number in a dosage of 50 mg/1 ml/day on the 21st days after treatment.
Conclusion: the extract of bitter melon fruit (Momordica charantia L.) at a dosage of 50 mg/kg/1ml/day can lower blood glucose levels and increased the number of Langerhans islets and Leydig cell of hyperglycemia mice.

Keywords: hyperglycemia, melon extract, blood sugar levels, pancreas cells, leydig cells

Citation: Meles DK, Wurlina, Anom Adnyana DP, Rinaldhi CP, Octaviani RR, Sekar Cempaka DK. The antidiabetic effect of bitter melon (Momordica charantia L.) extracts towards glucose concentration, langerhans islets, and leydig cells of hyperglycemic mice (Rattus norvegicus). Eurasia J Biosci. 2019;13(2):757-62.

Changes of metabolism in animals due to conditions of a purulent wound process while using photomineralisation as dressing tools
U. A. Krut, I. I. Oleynikova, A. I. Radchenko, E. V. Kuzubova

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Abstract

Annotation: the wound process is difficult, multicomponent and has its own a phase of character. Recently, there has been an active search for new products for the treatment and prevention of purulent wounds. The effect of sorbents on the process of wound healing is being studied. The impact of the damaging factor causes the development of inflammation, which leads to immediate biochemical changes. During primary alteration, cells are destroyed and their contents are released, the permeability of the vascular wall increases, and individual blood components can be easily transported to the external environment. Previously, a decrease in the concentration of total protein in the blood has already been noted. At the first stage of the wound process, one part of the protein is transferred to the exudate, and the other goes to decay to amino acids. In terms of energy disbalance amino acids enter into deamination and transamination reactions, as indicated by the high activity of alanine aminotransferase and aspartate aminotransferase as well as increased concentration of urea. The products of amino acid breakdown enter the Krebs cycle for subsequent energy production, which is necessary for further regeneration processes. Violations of the integrity of muscle tissue leads to the fact that the concentration of creatinine in the blood increases. Creatine phosphate due to the destruction of muscle fiber does not mobilize the generation of ATP, but reacts to non-enzymatic dephosphorylation with the formation of creatine anhydride - creatinine.

Keywords: wound process, metabolism, phytomineradsorbents, montmorillonite, biochemical changes, metabolism, inflammatory process

Citation: Krut UA, Oleynikova II, Radchenko AI, Kuzubova EV. Changes of metabolism in animals due to conditions of a purulent wound process while using photomineralisation as dressing tools. Eurasia J Biosci. 2019;13(2):763-7.

Combination of feed protein level and laserpuncture induction of broodstock catfish (Clarias sp.) to increase estrogen, vitellogenin, and egg quality
Dyah Hariani, Pungky Slamet WK

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Abstract

This study determined the combined impact of various dietary protein levels (30, 35, 40%) in adult catfish feed and laserpuncture induction on enhancing the estrogen and vitellogenin levels, and egg quality, based on the fertilization rate (FR), hatching rate (HR), and survival rate (SR) after spawning. The test fish were 8–9-month-old FI hybrids obtained by cross-breeding a mature Sangkuriang female with a mature Paiton male. In total, 172 female (900–1500 g body weight) and 172 male (1140–1750 g body weight) catfish were collected from UPBAT Kepanjen District, Malang, Indonesia. Fish fed diets with increased protein levels and exposed to laserpuncture induction had significantly enhanced estrogen and vitellogenin blood serum levels (P<0.001) in weeks 3 and 6 compared to 6 weeks for the negative control. Based on the egg quality data, the addition of 40% protein in the diet of the reproductively mature females together with laserpuncture induction produced the highest FR, HR, and SR (P<0.05) compared to protein levels 30 and 35%, and the negative control.

Keywords: Clarias sp., estrogen, fertilization rate, hatching rate, laserpuncture induction, protein level feed, survival rate, vitellogenin

Citation: Hariani D, Slamet WK P. Combination of feed protein level and laserpuncture induction of broodstock catfish (Clarias sp.) to increase estrogen, vitellogenin, and egg quality. Eurasia J Biosci. 2019;13(2):769-79.

Application of chitosan absorbent in reducing the amount of airport wastes containing ethylene glycol pollution
Pedram Azimi, Pirouz Derakhshi, Kambiz Tahvildari, Fereshteh Motiee

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Abstract

In this research, chitin and chitosan were used to remove the water pollutants and wastewater through adsorption process, due to their distinctive biological and physical-chemical characteristics, and their efficiency was investigated to remove ethylene glycol cationic pollutant from aqueous environments of wastewaters generated from airport. Using the ethylene glycol leads to the formation of wastes containing EG mixed with mineral salts. There are several methods for treating these wastes, in the research; we intend to investigate the efficiency of the adsorption phenomenon to remove ethylene glycol from industrial wastes. Non-continuous absorption experiments were conducted to examine the effect of the factors affecting the removal process, including the effect of contact time of absorbent with pollutant, PH, initial concentration of the pollutant and the amount of consumed absorbent. The results indicate that chitosan is suitable for absorbing ethylene glycol from industrial wastewaters, and by increasing absorbent weight, the absorption rate also increases.

Keywords: adsorption, airport wastewater, chitin, chitosan, ethylene glycol

Citation: Azimi P, Derakhshi P, Tahvildari K, Motiee F. Application of chitosan absorbent in reducing the amount of airport wastes containing ethylene glycol pollution. Eurasia J Biosci. 2019;13(2):781-9.

Rho-kinase inhibitor Y-27632 on Schlemm’s canal diameter in a juvenile rat model injected with sodium hyaluronate
Wino Vierlia Vrieda, Lely Retno Wulandari, M. Ma’sum Effendi, Hidayat Sujuti

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Abstract

Background: The purpose of this study was to examine the effects of the rho-kinase inhibitor Y-27632 on the diameter of the Schlemm’s canal in a juvenile rat model injected with sodium hyaluronate.
Material and Methods: This was an original experimental study with a post-test control group design. Eyeballs of rats aged 4-6 months were used in this study. Samples were divided into six groups: negative control, positive control I with intracameral sodium hyaluronate injection, positive control II with topical Y-27632 10 mM, and three experimental groups with intracameral injections of sodium hyaluronate and Y-27632 10-1 mM, 1 mM, and 10 mM, respectively. Changes of the Schlemm’s canal diameter were evaluated. Quantitative measurements were taken using computerized image analysis with the dot slide program.
Results: There were statistically significant differences among the control and experimental groups (p < 0.05). The greatest increase in diameter of the Schlemm’s canal was observed in the experimental group given sodium hyaluronate and Y-27632 10 mM, with a mean value of 118.42 µm.
Conclusion: The rho-kinase inhibitor Y-27632 increased the diameter of the Schlemm’s canal in juvenile rats injected with sodium hyaluronate.

Keywords: Juvenile rat model, rho-kinase inhibitor (Y-27632), Schlemm’s canal, sodium hyaluronate

Citation: Vrieda WV, Wulandari LR, Effendi MM, Sujuti H. Rho-kinase inhibitor Y-27632 on Schlemm’s canal diameter in a juvenile rat model injected with sodium hyaluronate. Eurasia J Biosci. 2019;13(2):791-4.

Histopathological and Biochemical study on the effect of flavonoids isolated from the plant Curcuma longa effective in liver enzymes (GOT, GPT) to female rats infected eggs diabetes induced in alloxan
Yasameen H. Jassim Al-samarrai, Adnan Majeed Mohammad Alsamarraie, Mohammed Abbas Fadhil, Buraq Mahmood Atta AL-badri

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Abstract

The study was conducted in Faculty of Applied Science Department of Applied Chemistry / University of Samarra the study included to recognize the effects of flavonoids isolated from a Curcuma longa plant to minimize the damage caused by diabetes induced by alloxan in the histological structure of liver and the levels of the (GOT & GPT) in liver congeneric. The experimental divided to three group each group contains five rats. The one group is control and the second group is adiabatic animal by peritoneal injection Alloxan 50 mg/kg according to body weight it has not been treated, the final group was the diabetic animals and it has been processed with Alloxan 50 mg/kg and flavonoids compound 40 mg/kg and in the final of the experiment the animal killed for anatomy liver and cut 2gm of it to do histological sections from Liver for the study and measurement the concentration of enzymatic liver GOT & GPT.

Keywords: flavonoids, alloxan, liver, GOT & GPT

Citation: Al-samarrai YHJ, Alsamarraie AMM, Fadhil MA, AL-badri BMA. Histopathological and Biochemical study on the effect of flavonoids isolated from the plant Curcuma longa effective in liver enzymes (GOT, GPT) to female rats infected eggs diabetes induced in alloxan. Eurasia J Biosci. 2019;13(2):795-801.

Pecularities of the influence of cyclophosphamide and imunophan on the processes of apoptosis and necrosis in Peyer’s patches of rats’ small intestine
Elena N. Morozova, Vitaliy N. Morozov, Alexey V. Tverskoi, Svetlana V. Zabolotnaya, Elena V. Kaliuzhnaya

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Abstract

In this article, we studied the peculiarities of cyclophosphamide and imunofan effect on the processes of apoptosis and necrosis in Peyer’s patches of the rats’ small intestine. The study was conducted on 18 white mature rats, which were divided into three groups. Group I was administered imunofan according to the scheme on days 1, 3, 5, 7, 9 of the experiment at a dose of 0.7 µg/kg of body weight of the animal, Group II was administered cyclophosphamide once at a dose of 200 mg/kg (for 1 day), then imunofan according to scheme (on the 2nd, 4th, 6th, 8th, 10th day of the experiment) at a dose of 0.7 µg/kg body weight of the animal, and Group III - intact animals served. Ultrathin sections of Peyer’s patches of the rats’ small intestine were prepared and studied under electron microscope with further photographing. Mostly one type of cell death - apoptosis is detected in group I animals on the 7th and 30th day of the experiment in Peyer’s patches. The number of cells with apoptosis is directly proportional to the observation period, which seems to indicate the ability of imunofan to influence this process, maintaining the constancy of Peyer’s patches cellular composition in small intestine. In animals of group II, on the 7th day of the experiment, both apoptosis and necrosis take place, and on the 30th day it is mainly apoptosis. Apparently, this is due to the immunosuppressive effect of cyclophosphamide on the lymphocytes of Peyer’s patches of the small intestine, which manifests itself in the early period of the experiment after correction by the immunomodulator.

Keywords: peyers’ patches, cyclophosphamide, imunofan, apoptosis, necrosis

Citation: Morozova EN, Morozov VN, Tverskoi AV, Zabolotnaya SV, Kaliuzhnaya EV. Pecularities of the influence of cyclophosphamide and imunophan on the processes of apoptosis and necrosis in Peyer’s patches of rats’ small intestine. Eurasia J Biosci. 2019;13(2):803-6.

Effect of Mitomycin C on human tenon fibroblast proliferation in contracted socket tissue
Mirza Metita, Debby Shintiya Dewi, Hidayat Sujuti

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Abstract

Background: This study aimed to evaluate the effects of mitomycin C on human tenon fibroblast proliferation in contracted socket tissue.
Material and Methods: Human tenon fibroblasts from a patient who underwent socket reconstruction surgery were cultured in vitro. Cell cultures were exposed to 0.2, 0.4, and 1 mg/ml of mitomycin C solution for 5 min, and serum-free culture medium was used as a control. Proliferation was observed with the MTT cell proliferation assay 24 h after treatment.
Results: A 5 min exposure to mitomycin C at 0.2, 0.4, and 1 mg/ml caused inhibition of fibroblast proliferation (p < 0.05). The dose of mitomycin C was not significantly related to the rate of fibroblast proliferation inhibition. Mitomycin C 0.4 mg/ml caused the greatest inhibition of fibroblast proliferation compared with doses of 0.2 and 1 mg/ml.
Conclusion: Mitomycin C can inhibit tenon fibroblast proliferation of contracted socket tissue. Dose escalation of mitomycin C is not related to the rate of fibroblast proliferation inhibition.

Keywords: contracted socket, mitomycin c, tenon fibroblast proliferation

Citation: Metita M, Dewi DS, Sujuti H. Effect of Mitomycin C on human tenon fibroblast proliferation in contracted socket tissue. Eurasia J Biosci. 2019;13(2):807-10.

Refractive error and its correlation with eye deviation as examined with the Hirschberg test in elementary and junior school students in Lesanpuro, Malang
Nanda Wahyu Anandita, Nafitri Aulia, Anny Sulistiyowati, Lely Retno Wulandari

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Abstract

Refractive error and eye deviation are two common pediatric eye conditions. Ophthalmic screening in children is essential for protecting against vision loss. In 2010, the WHO estimated that refractive error causes 42% of vision disturbances globally. Uncorrected refractive error can cause eye deviation. In this study, we report the characteristics of refractive error and its correlation with eye deviation, as examined with the Hirschberg test, in children from elementary and junior schools in Lesanpuro, Malang. This study involved 606 children aged 6-17 years, from elementary and junior schools. This was a cross-sectional observational descriptive study. Data was obtained from refraction examinations and the Hirschberg test. Each subject underwent one refraction test and one test of eye position. The results showed emmetropia, myopia, astigmatism, and hyperopia in 450 (74.26%), 131 (21.62%), 56 (9.24%), and 5 (0.83%) children, respectively. Eye deviation was found in six children (1.65%), all of which showed extropia (100%). Eye deviation occurred together with myopia in one child (17%), and with astigmatism in three children (50%). Two further children (33%) had other conditions such as amblyopia and corneal scar. Eye deviation is most common in astigmatism. For refractive errors, myopia is the most common condition while hypermetropia is the least common.

Keywords: eye drop, normal tension glaucoma, therapeutic compliance

Citation: Anandita NW, Aulia N, Sulistiyowati A, Wulandari LR. Refractive error and its correlation with eye deviation as examined with the Hirschberg test in elementary and junior school students in Lesanpuro, Malang. Eurasia J Biosci. 2019;13(2):811-5.

The RASA1 and Mir182 expression is reliable predictor for detection Acute Myeloid Leukemia
Akram Sadat Seyed Sharifi Kakhki, Khadijeh Onsory

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Abstract

Introduction: Acute myeloid leukemia (AML) is a common type of acute leukemia in adults. The RAS pathway dysregulation is one of the most common routes of the cancers and RASA1 gene family plays a key role on RAS deactivation. The Mir182 is involved in cancer cells proliferation and survival. So, the aim of the current study was to determine the expression of RASA1 and Mir182 in patients with AML using Real time PCR and compare it with control group.
Materials and Methods: For this purpose, the blood samples were collected from 60 AML patients and 30 healthy individuals as control. The RNA extracted, quality and quantity of the RNA determined, cDNA synthesized and primers were designed. Then genes expression investigated using Real time PCR and ∆∆CT computational techniques. Results analyzed using GraphPad Prism 6.0 software.
Results: According to the results, the RASA1 gene expression significantly decreased in the AML patients compared to the normal group (P<0.0001), while the expression of Mir182 increased in the AML compared to the healthy people (P<0.0001). No significant correlation was observed between age, sex, WBC, PLT, HGB, Blast and FAB subtype with the disease (P> 0.05).
Conclusion: These results suggested elevated expression of the Mir182 might suppress the RASA1 gene expression. So, determining of the RASA1 and Mir182 levels might be a useful indicator for prognosis of the AML.

Keywords: RASA1, Mir182, Acute Myeloid Leukemia, Real time PCR

Citation: Seyed Sharifi Kakhki AS, Onsory K. The RASA1 and Mir182 expression is reliable predictor for detection Acute Myeloid Leukemia. Eurasia J Biosci. 2019;13(2):817-22.

Effect of Spirulina platensis extract on Vascular Endothelial Growth Factor (VEGF) expression in corneal inflammation in rat (Rattus novergicus) strain wistar
Rosy Aldina, Shanti Widya Haryati

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Abstract

Background: Spirulina is a microalga known as has anti-inflammatory, anti-angiogenic, antioxidant, antibacterial and radioprotective properties. The aim of this study was to elucidate the differences in VEGF expression in response to various dosages of Spirulina platensis (SP) topical treatment in normal and corneal inflammation in the rat.
Material and Methods: This was an experimental study. SP powdered extract was obtained by maceration method using water as a solvent. Effect on VEGF expression was analyzed after SP aqueous extract topical treatment in corneal inflammation rat model for four times a day, over seven days. Corneal inflammation was induced by basic chemical trauma using 1N NaOH. VEGF expression was analyzed by histopathology: cornea samples were made into microscope slides and stained with immunofluorescent stain. Quantification was aided with confocal laser microscopy.
Results: Significant differences of VEGF expression were observed after topical treatment with 50 µg/mL, 100 µg/mL, and 200 µg/mL dosages. A significant correlation was also observed between SP treatment dosages and VEGF expression.
Conclusion: Topical SP treatments at 50 µg/mL, 100 µg/mL, and 200 µg/mL concentrations have anti-angiogenic effects by reducing VEGF expression in the cornea inflammation model in rats.

Keywords: cornea neovascularization, Spirulina platensis extract, Vascular Endothelial Growth Factor (VEGF)

Citation: Aldina R, Haryati SW. Effect of Spirulina platensis extract on Vascular Endothelial Growth Factor (VEGF) expression in corneal inflammation in rat (Rattus novergicus) strain wistar. Eurasia J Biosci. 2019;13(2):823-9.

Evaluation of the raw stock of giant ferula roots in the natural populations of the Mangystau desert
M. S. Sagyndykova, A. A. Imanbayeva, M. Yu. Ishmuratova

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Abstract

This paper presents the results of a study of the distribution and raw material stocks of a medicinal plant - giant ferula in the natural populations of the Mangyshlak desert. This species grows in the East Mangyshlak and South Mangyshlak geobotanical districts, in the southern part of the desert of the Mangyshlak Peninsula, covering the Tuyesu sands, loamy massifs in the vicinity of the Tynymbay shoky highland, sandy massifs in the vicinity of the Karkol wintering ground. According to the results of a survey of 6 natural populations of giant ferula (Tuyesu sands, outskirts of Tynymbay shoky, sands in the vicinity of Karkol wintering ground), the area of identified vegetation is estimated at 35 hectares, the operational stock of underground organs is estimated at 1029.6 cwt, the annual possible collection of raw materials is 102.9 cwt. Rational methods of collecting raw materials and exploitation of thickets have been developed: the same thickets can operate no more than 1 time in 2-3 years; raw materials harvested must include only individual plants that have entered the generative phase; the total amount of recoverable raw materials cannot exceed 10% of the operational stock.

Keywords: giant ferula, Mangyshlak, Western Kazakhstan, raw stock, productiveness, underground organs

Citation: Sagyndykova MS, Imanbayeva AA, Ishmuratova MY. Evaluation of the raw stock of giant ferula roots in the natural populations of the Mangystau desert. Eurasia J Biosci. 2019;13(2):831-8.

Protective effect of methanol extract of Kelor (Moringa oleifera) leaves on Glutathione Peroxidase (GPx) levels in trabecular meshwork cell culture of primary congenital glaucoma patients
Lely Retno Wulandari, Sri Umiati, Hidayat Sujuti

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Abstract

Background: The aim of this study was to determine differences in glutathione peroxidase (GPx) activity after multiple doses of methanol extract of Moringa oleifera leaves in a trabecular meshwork cell culture from primary congenital glaucoma patients.
Material and Methods: This was an experimental laboratory study; cell line cultures were divided into four groups (negative control and three treatment groups). The treatment groups were exposed to methanol extract of Moringa oleifera leaves at dosages of 15 µg/mL, 20 µg/mL, and 25 µg/mL. After four hours incubation, GPx activity was measured in all groups using a colorimetric microplate reader.
Results: ANOVA analysis found that there was a difference in GPx activity between treatment groups. Each Moringa oleifera extract dosage (15 µg/mL, 20 µg/mL, and 25 µg/mL) significantly differed from each other, with dosage 25 µg/mL having the highest GPx activity. The correlation analysis showed there was a significant association between exposure to Moringa oleifera extract and GPx activity with a positive correlation coefficient (r = 0.962, p < 0.005), which means higher dosages of Moringa oleifera extract will increase GPx activity. Linear regression analysis found thet every 1 mg/mL dosage of Moringa oleifera extract will increase GPx activity by 1.217 mU/mL.
Conclusion: It can be concluded that there were differences in GPx activity between treatment groups and there was an influence of dosage of methanol extract of Moringa oleifera leaves on GPx activity, such that increasing doses of methanol extract of Moringa oleifera leaves will further increase GPx activity.

Keywords: Gluthathione Peroxidase (GPx), methanol extract, Moringa oleifera leaves, primary congenital glaucoma

Citation: Wulandari LR, Umiati S, Sujuti H. Protective effect of methanol extract of Kelor (Moringa oleifera) leaves on Glutathione Peroxidase (GPx) levels in trabecular meshwork cell culture of primary congenital glaucoma patients. Eurasia J Biosci. 2019;13(2):839-44.

Biological resources of natural forage grassland of the cretaceous south of the European Russia
Vladimir I. Cherniavskih, Nicolay I. Sidelnikov, Elena V. Dumacheva, Zhanna A. Borodaeva, Tatyana N. Glubsheva, Anzhelika A. Gorbacheva, Oksana V. Vorobyova, Svetlana Korolkova

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Abstract

Problems of climatic changes and questions of the use of the natural resources for creation of a food security system are discussed. It demands an involvement in the agrarian production of the cretaceous South of the European part of Russia of local vegetable biological resources and of use for this potential of natural forage grasslands of the territory. The main objective of the made researches was assessment of territorial confinedness of economic and valuable plant species and also a status and productivity of a natural forage grassland within agrolandscapes of the cretaceous South of the European Russia (the territory of the Belgorod region). Biological and vegetable resources of the natural forage grasslands of the East part of the Belgorod region in a subband of the southern forest-steppe were studied. Field geobotanical inspections of large arrays of ravine and frame complexes and meadows, of the most perspective for the organization of haymaking and pasturable economy, were realized in 2012-2018. Research stations are located in Alexeyevsky district of the Belgorod region (tab time – 2002). The climate of the studied territory is moderately continental with a big annual amplitude of temperatures, a rather soft winter with a frequent thaw and snowfalls; with the solar and long summer; moderate unstable moistening with dominance of summer rainfall. It is established that in the steppe calciphilous communities long-term herbs prevail, and bushes and trees play the subordinated role. At decrease in degree of a pasture, intensity of mowing and recreational loading. The share of wood and shrubby vegetation increases. The overground phytomass of natural forage grasslands increases in process of remoteness from settlements and decreases in economic loading. The biggest value for the livestock production has communities with dominance of Bromopsis inermis, mixed herbs and the content of bean herbs of 15-25%. The received data about productivity and biological capacity of natural forage grasslands, allows to plan their share in the haying and pasturable line during cultivation of cattle. The oveground phytomass of communities slightly changed by years of a research. Communities with dominance of Bromopsis inermis and mixed herbs – on 50-68 g/m2 are higher than the others, have the largest phytoweight. The main phytomass of hay crops consists by the types having high growth rate: Bromopsis inermis, Diplotaxis cretacea, Festuca orundinacea, Lotus corniculatus, Matthiola fragrans, Medicago falcata. Perspective forage grasslands are located in the lower slope clots. Distribution of the types by the economic value showed that cereals make 10.8-47.2%, beans – 15.1-25.1% on weight of an absolute dry material. The correlation between productivity of the overground phytomass of communities and a projective covering (r=0.736) is discovered. The use of the studied territories as forage grasslands will allow to use rationally an arable land and to reduce the price of livestock production in the Belgorod region for 15-20%.

Keywords: biological resources, carbonate soils, natural forage grasslands, productivity of communities, economic and valuable plant species, cereal herbs, bean herbs

Citation: Cherniavskih VI, Sidelnikov NI, Dumacheva EV, Borodaeva ZA, Glubsheva TN, Gorbacheva AA, et al. Biological resources of natural forage grassland of the cretaceous south of the European Russia. Eurasia J Biosci. 2019;13(2):845-9.

Benign eyelid and conjunctival tumor profile in polyclinic RSSA July period 2012 - September 2015
Elfina Gatot Sadono, Yasmin Sani, Rosy Aldina

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Abstract

Tumors of the eye and adnexa tissue occur in 50% of all eye biopsies. This study aim to To determine the profile of benign primary tumors of the eye and characteristics of benign primary tumor patients at the Saiful Anwar Hospital, Malang. Data were obtained from the medial records of patients at the eye polyclinic, section of reconstruction, oculoplasty, and oncology, over a three-year period (July 2012 to September 2015). Patients were eligible for inclusion if they were diagnosed with benign eyelid or conjunctival tumors. Within three years (July 2012 to September 2015), a total of 307 patients were diagnosed with palpebral or conjunctival benign tumors. In total, 194 patients (63%) were female and 113 (36.8%) were male. The age of patients diagnosed with benign tumors ranged from two months to 80 years, with patients most commonly aged 10-30 years. The types of benign conjunctival tumors included: papilloma, nevus, lipoma, cyst, and granuloma. The types of benign palpebral tumors included; hemangioma cavernosa, hemangioma kapilare, cyst, nevus, xanthelasma, millia, granuloma, atheroma, lipoma, lithiasis, papilloma, hordeolum, and khalazion. Patients who attended the eye and tumor reconstruction section of the Saiful Anwar Hospital between July 2012 and September 2015 were mostly women, aged 10-29 years. Most benign tumors were located in the patient’s right eye. The most common palpebral tumor diagnosis was khalazion (133 patients), whereas the most common conjunctival tumor was a cyst (14 patients).

Keywords: benign conjunctival tumor, benign palpebral tumor

Citation: Sadono EG, Sani Y, Aldina R. Benign eyelid and conjunctival tumor profile in polyclinic RSSA July period 2012 - September 2015. Eurasia J Biosci. 2019;13(2):851-3.

Some of the features of the viscoplastic media
Marina Y. Nazarova, Vladimir V. Krasilnikov, Vitaly B. Nikulichev, Olga I. Matvienko, Nikolay V. Kamyshanchenko

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Abstract

The article considers the influence of the structure and the chemical composition of ground blast furnace slag on rheological properties of slag suspensions. Different composition and structure of blast furnace slags were studied. The features of structural and mechanical properties of suspensions are revealed at shear rate 1÷50c-1. The theoretical model of the flow in a flat endless channel at a final external pressure difference is presented on the basis of experimental data. The exact solution of the system of equations describing the considered flow is obtained.

Keywords: blast furnace slag, structure, chemical composition, rheological properties, quasi Bingham medium

Citation: Nazarova MY, Krasilnikov VV, Nikulichev VB, Matvienko OI, Kamyshanchenko NV. Some of the features of the viscoplastic media. Eurasia J Biosci. 2019;13(2):855-9.

Adoption of agricultural innovations in the context of zero waste: The case of dairy cattle biogas waste
Endang Yektiningsih, Penta Suryaminarsih, Ramdan Hidayat

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Abstract

The success of a sustainable agriculture program is determined by the farmers, who are the main actors. Zero waste technology started to be used a few years ago, but there is still little awareness among farmers about how to use it. A large number of animal husbandry households have used biogas energy sources for cooking through the utilization of dairy cattle manure. The use of these technologies still causes problems, because there is a great deal of liquid and solid waste every day. The waste from biogas processing can be converted into liquid and solid fertilizers. This study aims to analyze the factors that influence the adoption of biogas waste technological innovations by farmer groups. The variables that are assumed to affect the adoption of the technology are age, level of education, the managerial skills of the farmer group, level of confidence in the success of the technology, the technological needs, interest in product sales, and the amount of guidance from extension officers. The research sample consists of the members of farmer groups who were chosen using a random sampling method from two farmer groups in different locations. The research results showed that the factors affecting the adoption of innovations in biogas waste technology are the level of education, the level of confidence in the success of the technology, interest in the product sales, and amount of guidance from extension officers.

Keywords: adoption innovation, biogas waste technology, farmer groups

Citation: Yektiningsih E, Suryaminarsih P, Hidayat R. Adoption of agricultural innovations in the context of zero waste: The case of dairy cattle biogas waste. Eurasia J Biosci. 2019;13(2):861-4.

Current hydroecological situation of the Starooskolsko-Gubkinsky mining region on the example of the Oskolets River
Andrei G. Kornilov, Sergey N. Kolmykov, Alexander V. Prisny, Maria G. Lebedeva, Eugeniya A. Kornilova, Alexander A. Oskin

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Abstract

The results of the hydroecological study of the small Oskolets river in the area of influence of mining enterprises are presented. It is shown that the residential industrial flow of Gubkin city has a leading influence on the hydrochemical situation. Discharges of drainage waters made by the Lebedinsky mining and processing plant have a definite impact on the hydro-ecological situation with respect to the content of fluorine and lead compounds, and occasionally with respect to nitrogen compounds. The dynamics of hydrochemical indicators makes it possible to note trends related both to the production activities of the mining complex and the development of agricultural and industrial-urban infrastructure. In particular, there is an increase in the contribution of nitrogen, nickel, and sulphate compounds from the industrial and urban sector of Gubkin city, a slight increase in the supply of copper from residential and agricultural areas with periodic peak values of copper concentrations from the urban sector.

Keywords: hydrochemical indicators, hydroecological situation, ecology of the mining region, water pollution indicators

Citation: Kornilov AG, Kolmykov SN, Prisny AV, Lebedeva MG, Kornilova EA, Oskin AA. Current hydroecological situation of the Starooskolsko-Gubkinsky mining region on the example of the Oskolets River. Eurasia J Biosci. 2019;13(2):865-70.

Relationship between anthropometric parameters and dietary factors in refractive error in Indonesia
Nanda Wahyu Anandita, Nurul Aini

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Abstract

Background: Refractive error can be associated with nutrition status. This study aim to investigate the associations between anthropometric parameters and dietary factors in refractive error.
Material and Methods: Samples utilized were 252 eyes from 127 students between 13-17 years of age (mean 15 ± 0.67 y.o.). Seventy-five subjects were males and 52 were females. Subjects underwent visual acuity examination, noncycloplegic refraction, measurement of height and weight and each student was interviewed and filled out the semi-quantitative food frequency questionnaire (SQ-FFQ) to obtain daily nutrient intake.
Results: There was no significant association between age, weight, height, BMI and nutrient intake from SQ-FFQ (energy, protein, fat, carbohydrates, fibers, PUFA, cholesterol, vitamin A, carotene, vitamin E, vitamins B1, B2 and B6, folate, vitamin C, sodium, potassium, calcium, magnesium, phosphorus, iron and zinc) with SE (r < 0.2, p > 0.005).
Conclusion: In this study, there were no significant associations between anthropometric parameters and dietary factors with refractive error.

Keywords: anthropometric parameters, body mass index, dietary factors, food frequency questionnaire, height, NutriSurvey, refractive error, weight

Citation: Anandita NW, Aini N. Relationship between anthropometric parameters and dietary factors in refractive error in Indonesia. Eurasia J Biosci. 2019;13(2):871-5.

Monitoring of the content of manganese in soils and agricultural plants of the central Chernozem Region of Russia
Sergey V. Lukin, Denis V. Zhuykov, Ilya G. Kostin, Ekaterina A. Prazina, Aleksey A. Zavalin, Vladimir A. Chernikov

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Abstract

The paper deals with the analysis of long-term observations of the manganese distribution in the soils of the south-western part of the Central Chernozem region of Russia in the Belgorod region. The soil mantle of the survey area is represented mainly by typical chernozem soil, leached chernozem soil and common chernozem soil. It has been found according to the results of a continuous agrochemical survey that 56.4% of cultivated soils belongs to the category with moderate supply of movable manganese, and 38.7% - to the category with low supply. It has been noted that among the main legumes white lupine has a very high ability to absorb manganese. The average content of this metal in its grain is 1065 mg / kg, which is 44 times more than in soybeans and 120 times more than in peas. Among man-made sources of manganese in agrocenoses, organic fertilizers are the main ones.

Keywords: manganese, soil, chernozem, soybean, pea, white lupine

Citation: Lukin SV, Zhuykov DV, Kostin IG, Prazina EA, Zavalin AA, Chernikov VA. Monitoring of the content of manganese in soils and agricultural plants of the central Chernozem Region of Russia. Eurasia J Biosci. 2019;13(2):877-81.

Preparation of sodium-meta borate with series reactor and laboratory conditions
Masoud Shafiei, Piruz Derakhshi, Kambiz Tahvildari, Fereshteh Motiei

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Abstract

Sodium per-borate is obtained from the reaction between sodium-meta borate and hydrogen peroxide in the presence of magnesium sulfate or magnesium chloride as a stabilizer. Sodium-meta borate is obtained from reaction between sodium hydroxide and borax in the presence of sodium silicate. Typically this reaction is performed in a batch reactor. In this study, first, the reaction rate constant between sodium hydroxide and borax is obtained 4.94*10 (dm3/mole)2.12(sec)-1 , the reaction degree for sodium hydroxide was 2.47 and the reaction degree for borax was 0.65. Then continue using the obtained data in three series reactors with a volume of 1000ml for each operating conditions, respectively conversion percentage were in first reactor 41.6, second reactor 48.78 and the third reactor 50.96 while the percentage of computational conversion was respectively 84.79, 88.34 and 94.08. For obtaining the percentage of conversion in larger reactors respectively 1000, 1042 and 1110.

Keywords: sodium per-borate, sodium-meta borate, borax, sodium hydroxide, series reactors

Citation: Shafiei M, Derakhshi P, Tahvildari K, Motiei F. Preparation of sodium-meta borate with series reactor and laboratory conditions. Eurasia J Biosci. 2019;13(2):883-6.

The role of an angiopoietin-2 inhibitor in decreasing pericyte loss in diabetic rats
Nadia Artha Dewi, Dian Hapsari, Safaruddin Refa, Hidayat Sujuti

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Abstract

Background: The ratio of pericytes:endothelial cells in normal retinal vasculature is 1:1. Pericyte loss is the earliest morphological change in the diabetic retina and predominant characteristic in diabetic retinopathy. Diabetic retinopathy is associated with angiopoietin 2 (Ang-2). The aim of this study is to determine the role of an Ang-2 inhibitor in decreasing pericyte loss in diabetic rats.
Material and Methods: True experimental using rat model. Diabetic rats induced by intraperitoneal injection of streptozotocin (STZ). The samples were divided into 5 groups, injected intravitreally with vehicle (DMSO) or Ang-2 inhibitor with variation dose (10,20, and 30 μg). After one month the rat eyes were enucleated. Retinal digest preparation and HE staining was done to examine the retinal vasculature and count pericyte : endothel ratio.
Results: The number of pericytes reduced by 40% (1:2.5) after five weeks of diabetes induction in the diabetic rat model. The group receiving 10 μg Ang-2 inhibitor showed the best results in terms of reducing pericyte loss (1:1.8).The group receiving 20 μg Ang-2 inhibitor had a pericyte:endothelial ratio of 1:2.1. The pericyte loss was not decreased in the group receiving 30 μg Ang-2 inhibitor (1:2.8). Linear regression analysis revealed that there was a positive relationship between administration of the Ang-2 inhibitor and a reduction in pericyte loss in diabetic rats (p > 0.05).
Conclusion: Intravitreal administration of an Ang-2 inhibitor, at certain doses, reduced pericyte loss in diabetic rats.

Keywords: Angiopoietin2, pericyte loss, diabetic retinopathy

Citation: Dewi NA, Hapsari D, Refa S, Sujuti H. The role of an angiopoietin-2 inhibitor in decreasing pericyte loss in diabetic rats. Eurasia J Biosci. 2019;13(2):887-91.

Influence of palm oil enriched diet on the morpho-functional condition of rat’s mandibular condylar cartilage
Evgeniy N. Krikun, Kamilya R. Ismailova, Vladyslav I. Luzin, Vitaliy N. Morozov, Elena N. Morozova

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Abstract

Currently, it is known that saturated fatty acids in palm oil have a negative effect on the body: they activate the thrombus formation process, increase blood cholesterol levels and develop other metabolic disorders, which leads to the development of cardiovascular and oncological diseases, and also to dysfunction of the organs of the gastrointestinal tract. The aim of the study was to establish the morpho-functional condition of mandibular condylar cartilage in white rats of different ages under conditions of excessive use of palm oil and to assess the possibility of correcting the identified changes using the Garcinia Cambogia extract. The study was conducted on 216 white rats of three age groups: immature, mature and senile, divided into 3 groups. 1st group - control; 2nd group - animals treated with palm oil at the rate of 30 g / day / kg; Group 3 - rats, which after 6 weeks of administering palm oil began to receive Garcinia Cambogia extract at a rate of 0.25 g / kg body weight. The periods of observation were 1, 10, 30 and 60 days after the end of 6 weeks from the start of palm oil use. The histologic slides of mandibular condylar cartilage were made according to standard methods. The total width of the condylar cartilage of the lower jaw, the width of its individual zones, the volume content of primary spongiosa, and the number of cells in the zone of subchondral osteogenesis were measured on the sections obtained. The use of palm oil for 6 weeks at the rate of 30 g / day / kg is accompanied by inhibition of the osteogenic function of the condylar cartilage of the lower jaw of experimental animals, which increases with the duration of addition of palm oil to the diet. On day 1 of observation, the maximum in amplitude deviations are observed in immature animals, the minimum - in senile animals. In the future, the rate of increase of changes are maximum in senile animals. The introduction of the Garcinia Cambogia extract at the rate of 0.25 mg / day / kg starting from the 7th week of using palm oil is accompanied by smoothing the negative influence of the experimental conditions on the osteogenic function of the condylar cartilage from 10 to 60 days of observation in immature and mature rats and from 30 to 60 days in senile rats.

Keywords: rat, mandible, condylar cartilage, palm oil, Garcinia Cambogia extract

Citation: Krikun EN, Ismailova KR, Luzin VI, Morozov VN, Morozova EN. Influence of palm oil enriched diet on the morpho-functional condition of rat’s mandibular condylar cartilage. Eurasia J Biosci. 2019;13(2):893-7.

Inhibitory effect and preliminary phytochemical screening of some ornamental plants against some bacteria pathogens
Fred Coolborn Akharaiyi, Oke-Oghene Onyinnoyi Odiedi, Jonathan Abidemi Johnson, Funmilayo Stella Oluwafemi

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Abstract

Background: Bacterial resistance to the drugs known for their treatment is on the increase, therefore suggesting the need to search for dependable natural products as alternative for cure and prevention. Efforts in this regard have focused on plants because of their use historically and the large portions of the world’s population relying on plants for the treatment of infectious and non-infectious diseases.
Methods: Some ornamental plants’ extracts in compares with commercial antibiotics were tested in vitro on Salmonella typhi, Salmonella typhimurium, Staphylococcus aureus and Staphylococcus epidermidis for medicinal values. Quantitative and qualitative phytochemicals; free radical scavenging capacity, ferric reducing antioxidant property and hydroxyl radical scavenging of the plants’ extracts were determined by chemical methods.
Results: Varied degrees of inhibition and in some cases resistance were observed with the extracts on the four bacteria species. However, highest inhibition of 30.33±0.35 mm was recorded on Salmonella typhimurium, followed by S. epidermidis with zone of 30.00±0.00 mm both with H. crepitans extract. Cotrimozazole most inhibited Salmonella typhimurium with a zone of 29 mm and followed by ofloxacin with a zone of 27.33 mm on S. epidermidis. MIC activity of the extract was between 12.5 – 50 mg/ml and MBC activity from 25 – 100 mg/ml.
Conclusions: Valuable antibacterial effect of the plants’ extracts correlating with the phytochemicals and antioxidants potentials, suggest the plants acceptability for folklore and could be of universal recognition for handling diseases that plaque around us mostly in some urban and rural communities where modern medicine are not affordable and accessible by the poor.

Keywords: antibacterial, MIC, ornamental plants, chemicals, disease

Citation: Akharaiyi FC, Odiedi OO, Johnson JA, Oluwafemi FS. Inhibitory effect and preliminary phytochemical screening of some ornamental plants against some bacteria pathogens. Eurasia J Biosci. 2019;13(2):899-08.

Survival of medical leeches after partial cannibalism
Ruslan Aminov

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Abstract

In the experiment, 500 medical leeches, three types: H. verbаna, H. medicinalis, H. orientalis. The experiment was carried out for half a year, on leeches of middle and mother age. The leeches were set aside by 8-10 pieces in a tank with water after blood feeding, the survival rate after the manifestation of partial cannibalism was investigated. As a result, we found that the survival after all wounds in all three types was about 35%. After the wounds had healed, survivors of leeches developed constrictions at the wound site, which remained with them for life. It should be noted that partial cannibalism was observed in all three types of medicinal leeches, as well as in one species, and between species.

Keywords: survivability of medical leeches, partial cannibalism, hematophagus, blood, ectoparasite

Citation: Aminov R. Survival of medical leeches after partial cannibalism. Eurasia J Biosci. 2019;13(2):909-12.

Control of pests in the green mustard plant through papaya leaf extract
Yakobus Bustami, Florentina Rahayu Esti Wahyuni, Didin Syafruddin, Mulyono

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Abstract

A green mustard plant (Brassica juncea L) is often attacked by plant pests’ organisms, which often disturb farmers. This research aims to determine the types of pests and the effect of papaya leaf extract as a vegetable pesticide on pests in the green mustard plant. The research method used was an experiment with a completely randomized design consisting of 5 treatments and 5 replications with each concentration of papaya leaf extract as well as 0%, 25%, 50% 75% and 100%. The parameters observed were the types of pests and the impact of papaya leaf extract as vegetable pesticides on pests in the green mustard plant. The data collection was using observation sheets and documentation. The data analysis used descriptive and inferential analysis. The inferential analysis used the ANOVA test and continued with the Least Significance Different (LSD) test at a significant level of 0.05. The results show that there were several types of pests such as small snails, green grasshoppers (Atractomorpha crenulata), armyworms (Spodoptera litura), green caterpillars or leaf destroyers (Plutella xylostella). The results of statistical analysis showed that the administration of papaya leaf extract had a significant effect on pests that attack the green mustard plant. The results of the variance analysis showed a significant value of treatment (sig: 0.000) smaller than the alpha value (α: 0.05). Furthermore, the LSD test showed that the fifth treatment with 100% concentration was significantly different from other treatments in controlling pests in the green mustard plant.

Keywords: plant pests, green mustard plant, papaya leaf extract

Citation: Bustami Y, Esti Wahyuni FR, Syafruddin D, Mulyono. Control of pests in the green mustard plant through papaya leaf extract. Eurasia J Biosci. 2019;13(2):913-9.

Characterization of plant growth promoting rhizobacteria isolated from the rhizosphere of carob tree (Ceratonia siliqua L.) in Morocco
Rahal El Kahkahi, Meryama Moustaine, Souhail Channaoui, Majida Hafidi, Rachid Zouhair, Mustapha Ait Chitt, Rafik Errakhi

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Abstract

Plant growth promoting rhizobacteria (PGPR) are beneficial bacterial that colonize plant roots and enhance plant growth by a wide variety of mechanisms. Seven isolates of bacteria were successfully isolated and phenotypically, biochemically, physiological and molecular characterized. Subsequently to investigate the effect of PGPR isolates on the growth of carob tree, a pot culture experiement was conducted as Centre for Innovation and Technology Transfer (CITT), Meknes, Morocco. All the isolates were gram negative, rods, motile, able to grow on YDC medium and citrate simmons positive, degrade glucose, fructose and mannitol, capable of hydrolyzing esculin and starch. All strains are able to produce (74-150µgml-1) of indole acetic acid, nitrogen fixatin and all isolate are unable to produce ammonia, chitinase and cellulase, the strains 2018-3, 2018-4 and 2018-5 showed protease activity. The pecentage of solibilization of phosphate varied between 15% to 45%, the maximum phosphate solubilization was identified by stain 2018-4. Sequencing of 16SrRNA genes led to the identification of two family of bacteria : Bacillaceae and Pseudomonadaceae. The species of bacteria are : Bacillus thuringiensis, two of Bacillus flexus, two of Pseudomonas gessardii, Bacillus cereus and Bacillus thaonhiensis. The analysis the result showed that the highest vegetative growth was obtained from stain 2018-4 such as plant height (13.6cm), root length (13.65cm), fresh weight of the aerial part (1.09g) and fresh weight of the root part (0.42g) in comparison to untreated plant.

Keywords: carob tree, PGPR, IAA, P-solubilization, N2- fixation and plant inoculation

Citation: El Kahkahi R, Moustaine M, Channaoui S, Hafidi M, Zouhair R, Ait Chitt M, et al. Characterization of plant growth promoting rhizobacteria isolated from the rhizosphere of carob tree (Ceratonia siliqua L.) in Morocco. Eurasia J Biosci. 2019;13(2):921-30.

Farmer’s behavior towards Lembah Palu shallot farm risks in Central Sulawesi, Indonesia
Erny, Dwidjono Hadi Darwanto, Masyhuri, Lestari Rahayu Waluyati

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Abstract

The land in Central Sulawesi, particularly in Sigi Regency, is still quite large to cultivate shallots. With suitable rainfall and temperature as well as adequate facilities and infrastructure, the variety of shallots planted by farmers in Sigi Regency is Lembah Palu variety (local). Lembah Palu shallots are suitable to be processed as fried shallots because, in addition to having distinctive aroma and taste, they remain dry/crispy although stored for a long time. With such potential, it is necessary to make efforts to increase Lembah Palu shallot production. In fact, low production and quality of farm yields have made it difficult to gain maximum profits. In addition to considering the profits to gain, farmers also pay attention to any potential farm risks. (A high production risk will affect farmers’ behavior in decision making). This study aimed to reveal farmers’ behavior towards Lembah Palu shallot farm risks. The study was conducted in February to May 2018 at the center of Lembah Palu shallot production in Sigi Regency. The data analysis was done using a quantitative descriptive approach. Production risk was analyzed using multiple linear regressions with Cobb-Douglas production function as well as Just and Pope production function; Farmers’ behavior was analyzed using Khumbakar mathematical model. The results showed that 69.68% of the behavior of Lembah Palu shallot farmers in Sigi Regency was risk averse and 30.32% as risk takers. Recommendation. In order to gain higher yields, farmers should be risk takers.

Keywords: behavior, risk level, production, utility function

Citation: Erny, Darwanto DH, Masyhuri, Waluyati LR. Farmer’s behavior towards Lembah Palu shallot farm risks in Central Sulawesi, Indonesia. Eurasia J Biosci. 2019;13(2):931-6.

Agrotechnics of the soybean plant, which is sown as a secondary crop, effects of sowing period to the harvest and fertility characteristics of the seeds
Salavatova Khurshida

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Abstract

This article highlights the information on the importance of soybean plant as a secondary crop for soil fertility, agro technics of sowing soybean, the effects of soybean during sowing, and the growth of seeds when processing soybean with oxyhumate before sowing.

Keywords: soil, fertility, soybean, protein, humus, organic substances, oxyhumate, collection, spider worm, carcinoma, phosphorus and potassium fertilizers, bundle bacteria

Citation: Khurshida S. Agrotechnics of the soybean plant, which is sown as a secondary crop, effects of sowing period to the harvest and fertility characteristics of the seeds. Eurasia J Biosci. 2019;13(2):937-40.

Assessing genetic diversity for drought and heat stress tolerance of Nepalese wheat genotypes by SSR markers
Mukti Ram Poudel, Surya Kant Ghimire, Madhav Prasad Pandey, Krishna Hari Dhakal, Dhruba Bahadur Thapa, Deepak Kumar Khadka

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Abstract

The productivity of wheat in Nepal is low compared to the world average and very low compared to countries like New Zealand and Ireland, and it has remained nearly stagnant in the past ten years. Genetic factors, water and climate seem to be a limiting factor for wheat productivity in Nepal. Breeding for tolerance to biotic and abiotic stress and study of their genetic stability under stress are important for improving the productivity of wheat. We assessed the genetic diversity of 20 genotypes of wheat released or in the pipeline in Nepal, by 12 SSR markers linked to drought tolerance and 4 SSR markers linked to heat stress tolerance. The first set clustered 20 genotypes into 4 clusters, 3 of which further sub clustered into 8 sub clusters. 2 of the genotypes, namely BL-4707 and NL-1325, are distantly related to the rest of the genotypes. The second set of SSR markers clustered 20 genotypes into 5 clusters, 2 of which further subclustered into 4 subclusters. 2 of the genotypes, namely NL-1247 and NL-1325, are distantly related to each other and the rest of the genotypes. This study identified both closely related genotypes and distantly related genotypes of wheat in alleles presumably linked to drought and heat stress tolerance. The finding of this study is expected to be useful for breeding for drought and heat stress tolerance and study of the interaction of genotypes and environment as well.

Keywords: heat stress, drought, SSR, wheat, breeding, productivity

Citation: Poudel MR, Ghimire SK, Pandey MP, Dhakal KH, Thapa DB, Khadka DK. Assessing genetic diversity for drought and heat stress tolerance of Nepalese wheat genotypes by SSR markers. Eurasia J Biosci. 2019;13(2):941-8.